高效液相色谱法同时测定女金胶囊等四种中成药中橙皮苷和丹皮酚的含量  

Simultaneous determination of Hesperidin and Paeonol in Nujin capsule and other four Chinese patent medicines by high performance liquid chromatography

作　　者：李燕 同晓霞 杨晓莉 LI Yan;TONG Xiaoxia;YANG Xiaoli(TBioassay room,Yan’an Institute of Drug control,Yan’an,Shaanxi 716000,China;Microbiology room,Shaanxi Institute of Food and Drug Supervision and Inspection,Xi’an,Shaanxi 710065,China)

机构地区：[1]陕西省延安市药品检验所生测室,陕西延安716000 [2]陕西省食品药品监督检验研究院微生物室,陕西西安710065

出　　处：《安徽医药》2019年第12期2370-2373,共4页Anhui Medical and Pharmaceutical Journal

摘　　要：目的建立一种利用高效液相色谱法同时检测女金胶囊、调经丸、舒尔经颗粒、活血止痛膏四种中成药中橙皮苷和丹皮酚含量的方法。方法以女金胶囊为例,取供试品10粒,内容物混匀,精密称取0.2 g,置50 mL量瓶中,超声提取,甲醇定容;采用Agilent ZORBAX SB C18(4.6 mm×250 mm,5μm)色谱柱进行分离,0.2%磷酸溶液与甲醇梯度洗脱,柱温30℃,紫外检测器定量检测。结果橙皮苷和丹皮酚在质量浓度为0.2~20.0μg/mL范围内线性关系良好;相关系数R2>0.995;检测限:橙皮苷为0.05μg/mL、丹皮酚为0.08μg/mL;橙皮苷和丹皮酚3个浓度点(0.2、1.0和10μg/mL)回收率分别在92.6%~100.9%、90.6%~98.2%之间;方法重复性(n=6)在5.0%以内。结论该方法具有前处理简单、检出限低、准确度高等优点,可用于女金胶囊等四种中成药中橙皮苷和丹皮酚的含量的测定。Objective To establish a method for simultaneous determination of Hesperidin and Paeonol in Nujin capsule,Tiaojing pill,Shuerjing granule and Huoxue Zhitong ointment by high performance liquid chromatography.Methods Taking Nujin capsule as an example,10 test samples were taken,the contents were mixed,0.2 g was precisely weighed and placed in a 50 mL measuring bottle,extract by ultrasound,and fix the volume with methanol;agilent ZORBAX SB C18(4.6 mm×250 mm,5μm)column was se lected for separation,and the gradient was eluted with 0.2%phosphoric acid solution and methanol.Column temperature was main tained at 30℃,UV detector was selected for quantitative detection.Results Hesperidin and Paeonol showed good linear relation ship in the range 0.3 20.0μg/mL(R2>0.995).Detection limit of Hesperidin and Paeonol was 0.05μg/mL and 0.08μg/mL,respec tively.The recovery rates of Hesperidin and Paeonol at three concentration points(0.2,1.0,10μg/mL)were 92.6% 100.9%and 90.6% 98.2%respectively;the method repeatability of Hesperidin and Paeonol was both within 5.0%(n=6).Conclusion The method has the advantages of simple pretreatment,low detection limit and high accuracy.It can be used for the determination of Hesperidin and Paeonol in four Chinese patent medicines such as female gold capsules.

关 键 词：色谱法 高压液相 二极管阵列检测器 中成药 橙皮苷 丹皮酚 女金胶囊 调经丸 舒尔经颗粒 活血止痛膏 

分 类 号：R28[医药卫生—中药学]