强酸性电解质水对饥饿态粪肠球菌生物膜的杀灭作用研究  

Investigation of the killing effect of strong acid electrolytic water on biofilm of enterococcus faecalis in starvation phase

作　　者：赵春苗[1,2] 程小刚 余擎 ZHAO Chunmiao;CHENG Xiaogang;YU Qing(Department of Operative Dentistry and Endodontics,Stomatology Hospital of Baoji,Baoji,Shaanxi 721006,China;School of Stomatology of Air Force Medical University,Xi’an,Shaanxi 710032,China)

机构地区：[1]宝鸡市口腔医院牙体牙髓病科,陕西宝鸡721006 [2]空军军医大学口腔医学院牙体牙髓病科,陕西西安710032

出　　处：《安徽医药》2019年第12期2424-2427,共4页Anhui Medical and Pharmaceutical Journal

基　　金：2018年度宝鸡市卫生健康委员会计划立项课题(2018-49)

摘　　要：目的观察强酸性电解质水(SAEW)对饥饿态粪肠球菌生物膜的杀菌作用。方法将1.5×109 CFU/mL的粪肠球菌4℃低温离心、PBS溶液溶解,饥饿诱导形成饥饿态粪肠球菌生物膜,用SAEW、5.25%次氯酸钠(阳性对照)和0.9%氯化钠溶液(NS,阴性对照)分别处理1、5、10 min后,分别用活菌计数法和扫描电子显微镜(SEM)两种方法观察SAEW对饥饿态粪肠球菌生物膜的杀灭作用。结果活菌计数法显示SAEW对饥饿态粪肠球菌生物膜中的细菌表现出了有效的杀灭作用,且处理10 min时的细菌存活率(5.31±0.74)%与5.25%次氯酸钠的(4.06±1.44)%差异无统计学意义(P>0.05),即杀菌作用接近。SEM观察结果显示,随着NS处理时间的延长细菌细胞皱缩失水现象加重;SAEW和5.25%次氯酸钠处理后不仅有严重的皱缩失水现象,还出现了细菌细胞的溶解和破坏、细胞膜表面有穿孔和碎片样改变,而且随着处理时间的延长细菌细胞的破坏更加严重。结论SAEW对饥饿态粪肠球菌生物膜具有有效的杀灭作用。Objective To evaluate the bactericidal effect of strong acid electrolytic water(SAEW)on biofilm of enterococcus faeca lis in starvation phase.Methods Enterococcus faecalis dilution(1.5×109 CFU/mL)was centrifuged at 4℃and dissolved in PBS solution.Enterococcus faecalis in starvation phase were incubated to develop bacterial biofilms.Then the biofilms were individually treated with SAEW,sodium hypochlorite(NaClO),and normal saline for 1,5,and 10 min,respectively.Colony formation units(CFUs)counts and scanning electron microscopy(SEM)were used to investigate the bactericidal efficiency of SAEW on starved enterococcus faecalis biofilm.Results SAEW showed an effective killing effect on starved enterococcus faecalis biofilm.There was no significant difference in bacterial survival rate between SAEW(5.31±0.74)%and 5.25%NaClO(4.06±1.44)%at 10 min(P>0.05).That is,the killing effect is close.The results of SEM observation showed that the shrinkage and water loss of bacterial cells increased with the prolongation of NS treatment time.After treatment with SAEW and 5.25%NaClO,there was not only seri ous shrinkage and water loss,but also dissolution and destruction of bacterial cells,perforation and fragment changes on the surface of cell membrane,and the destruction of bacterial cells became more serious with the prolongation of treatment time.Conclusion SAEW has effective bactericidal efficiency to starved enterococcus faecalis biofilm.

关 键 词：粪肠球菌 生物膜 饥饿 次氯酸 电解 根管冲洗剂 强酸性电解质水 杀菌作用 

分 类 号：R44[医药卫生—诊断学]