紫丁香花精油的抗氧化和抗肿瘤活性研究  

Study on Antioxidant and Antitumor Activity of Essential Oil from Flowers of Syringa oblata

作　　者：胡建燃 李平 铁军[1] 金山[1] HU Jian-ran;LI Ping;TIE Jun;JIN Shan(Changzhi Univeristy,Changzhi 046011)

机构地区：[1]长治学院

出　　处：《生物技术通报》2019年第12期16-23,共8页Biotechnology Bulletin

基　　金：国家级高等学校大学生创新创业训练计划项目(201710122004);国家自然科学基金青年科学基金项目(31701125);山西省“1331工程”重点学科建设计划

摘　　要：采用水蒸气蒸馏法提取紫丁香花蕾精油,用3种不同的抗氧化体系检测了精油的抗氧化活性。测定不同质量浓度的紫丁香花精油溶液对1,1-二苯基-2-三硝基苯肼(1,1-diphenyl-2-picrylhydrazyl,DPPH)自由基、羟自由基的清除效果,并测定了其总抗氧化能力(Total antioxidant capacity,T-AOC)。结果显示,精油具有较好的DPPH自由基、羟自由基的清除作用,并且与精油浓度呈现出量效关系。精油的T-AOC是19.86±1.74 U/mg,高于化学抗氧化剂2,6-二叔丁基对甲酚(3,5-Di-tert-butyl-4-hydroxytoluene,BHT()0.38±0.08 U/mg)。精油的抗肿瘤活性检测采用胃癌细胞株MGC80-3和HGC-27,3(-4,5-二甲基-2-噻唑基)-2,5-二苯基四氮唑溴化物(3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide,MTT)法测定紫丁香花精油对癌细胞的抑制作用。TUNEL法检测精油对胃癌细胞凋亡的影响。MTT法检测结果证明紫丁香花精油可显著抑制两种胃癌细胞生长,其抑制作用与精油浓度呈正相关,MGC80-3和HGC-27细胞经精油处理48 h后的IC50值分别为0.282 mg/mL和0.263 mg/mL。利用TUNEL法检测细胞凋亡情况,结果显示,凋亡细胞数量随精油浓度的提高而显著增多,并且HGC-27细胞对紫丁香花精油更敏感。研究结果表明,紫丁香花精油具有良好的体外抗氧化和抗肿瘤活性,具备进一步药用研究和开发的价值。Essential oil in the flowers of Syringa oblata at the bud stage was extracted by hydro distillation,and 3 different antioxidant tests were employed to evaluate the antioxidant activities of the essential oil.The scavenging effects of the essential oil at different concentrations on DPPH radical and hydroxyl free radicals were measured,and the total antioxidant capacity(T-AOC)of the essential oil was also measured.The results indicated that the essential oil had a favorable scavenging effect on DPPH free radical and hydroxyl radical in a dose-dependent manner.The T-AOC of the essential oil was 19.86±1.74 U/mg,higher than that of 3,5-Di-tert-butyl-4-hydroxytoluene(0.38±0.08 U/mg).Gastric cancer lines MGC80-3 and HGC-27 were used to access the inhibitory effect of the essential oil by MTT(3-(4,5-Dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide)method.The apoptotic index of the essential oil was detected by TUNEL assay.The results of MTT assay indicated that the essential oil significantly inhibited MGC80-3 and HGC-27 in a dose-dependent manner,and the IC50 values,after 48 h exposure,were 0.282 mg/mL and 0.263 mg/mL respectively.TUNEL assay indicated the essential oil induced apoptosis also in a dose-dependent manner,and HGC-27 cells showed more sensitive than MGC80-3 cells.Therefore,essential oil in the flowers of S.oblata shows remarkably high antioxidative and antitumor activities in vitro,and demonstrated potential value of medical application.

关 键 词：紫丁香花 精油 抗氧化 抗肿瘤 细胞凋亡 

分 类 号：R28[医药卫生—中药学]