维药洋甘菊化学成分及DPPH自由基清除活性研究  

Chemical constituents in flowers from Matricaria chamomilla L. and its scavenging activity of DPPH free radicals

作　　者：叶琦 汪洋[1] 李思婵 梅艳[1] YE Qi;WANG Yang;LI Si-chan;MEI Yan(Department of Pharmacy,Wuhan Children′s Hospital,Tongji Medical College,Huazhong University of Science&Technology,Wuhan 430000,China)

机构地区：[1]华中科技大学同济医学院附属武汉儿童医院

出　　处：《天然产物研究与开发》2019年第11期1907-1911,共5页Natural Product Research and Development

基　　金：国家自然科学基金青年基金(81600123)

摘　　要：为对洋甘菊(Matricaria chamomilla L.)的化学成分进行分离纯化及化合物DPPH自由基清除的活性进行考察,本实验通过正、反相硅胶、Sephadex LH-20、半制备高效液相色谱,从洋甘菊中分离得到11个化合物,通过波谱方法进行鉴定结构为山萘酚-3-O-β-D-葡萄糖苷(1)、木犀草素-3′-O-β-D-葡萄糖苷(2)、6-羟基木犀草素-7-O-β-D-葡萄糖苷(3)、异槲皮苷(4)、5-羟基-4′,7-二甲基-6,8-二甲氧基黄酮(5)、槲皮素-3′-O-β-D-葡萄糖苷(6)、3-羟基苯甲醇(7)、1-(4-羟基苯基)乙烷-1,2-二醇(8)、2-(3-甲氧基-4-羟基苯基)-丙烷-1,3-二醇(9)、2-(4-羟基乙基)乙醇(10)、丁香酸(11),其中化合物1~3、5、7~11为首次从该植物中分离得到,经DPPH清除自由基活性筛选发现化合物1~3、5、6有着与对照组相当的DPPH自由基清除活性。This paper reported the chemical constituents and their activity of scavenging DPPH free radicals in the flower of Matricaria chamomille.Eleven compounds were isolated from Matricaria chamomille by chromatography of silica gel,ODS,Sephadex LH20 and pre-HPLC,and their structures were elucidated by chemical properties and spectroscopic analyses.Their structures were elucidated as kaemperol-3-O-β-D-glucopyranoside(1),luteolin 3′-O-β-D-glucopyranoside(2),6-hydroxyluteolin-7-O-β-D-glucoside(3),isoquercitrin(4),5-hydroxy-4′,7-dimethoxy-6,8-dimethylflavone(5),quercetin-3′-O-β-D-glucoside(6),3-hydroxy benzenemethanol(7),1-(4-hydroxyphenyl)ethane-1,2-diol(8),2-(3-methoxy-4-hydroxyphenyl)-propane-1,3-diol(9),2-(4-hydroxyphenyl)ethanol(10),p-Hydroxybenzoic acid(11).Among which compounds 1-3,5,7-11 obtained from this plant for the first time.After DPPH radical scavenging assay,compounds 1-3,5 and 6 exhibited signficant activity.

关 键 词：洋甘菊 化学成分 自由基清除活性 DPPH 

分 类 号：R284.1[医药卫生—中药学]