紫苏叶挥发油舒张血管作用及其活性物质探究  

Study on the vasodilatory activity of volatile oil from Perilla leaves and its active substances

作　　者：周勤梅[1,2] 谯明鸣 彭成 敖慧[1] 熊亮 刘菲[1,2] ZHOU Qin-mei;QIAO Ming-ming;PENG Cheng;AO Hui;XIONG Liang;LIU Fei(Pharmacy College,Chengdu University of TCM,The Ministry of Education Key Laboratory of Standardization of Chinese Herbal Medicine,The State Key Laboratory of Characteristic Chinese Medicine Resources in Southwest China;Institute of Innovative Medicine Ingredients of Southwest Specialty Medicinal Materials,Chengdu University of Traditional Chinese Medicine,Chengdu 611137,China)

机构地区：[1]成都中医药大学药学院教育部中药材标准化重点实验室西南特色中药资源国家重点实验室 [2]成都中医药大学,西南特色药材创新药物成分研究所,成都611137

出　　处：《天然产物研究与开发》2019年第11期1949-1953,2000,共6页Natural Product Research and Development

基　　金：四川省青年科技创新研究团队专项(2017TD0001);成都中医药大学青年教师创新项目(ZRQN2018015)

摘　　要：为探究紫苏叶挥发油及其代表性成分对KCl预收缩胸主动脉血管环的影响,本文应用大鼠离体胸主动脉环试验对紫苏叶挥发油进行药效学评价,利用离体组织灌流模型和PowerLab数据分析系统采集和记录不同浓度紫苏叶挥发油对氯化钾(KCl)刺激下血管张力的影响。应用气质联用(GC-MS)技术对紫苏叶油进行化学成分分析,结合其代表性成分进行活性追踪,以阐释紫苏油舒张血管的药效物质基础。结果发现紫苏叶挥发油具有显著的舒张KCl预收缩胸主动脉血管环作用,其EC 50为8.6μg/mL,经GC-MS分析表明发挥作用的物质主要为单萜类成分,且发现其代表性成分紫苏醛具有一定的舒张血管作用。本研究不仅首次发现紫苏叶挥发油具有舒张血管活性,同时证实紫苏醛是紫苏叶油发挥舒张血管的药效物质基础之一,为紫苏叶的研究与开发提供新的依据。To investigate the effect of volatile oil and its representative components on the vessels under tension stimulated with potassium chloride(KCl)from Perilla leaves.The pharmacodynamic effects of volatile oil were evaluated by rat aortic rings,which were collected and recorded by the tissue perfusion model and the PowerLab data analysis system.The components of the essential oil obtained by hydrodistillation were investigated by GC-MS.And the representative compound was further studied in order to explain the pharmacodynamic basis of vasodilatation of perilla oil.The volatile oil exhibited significant activities on KCl-indued vascular rings,with EC 50 values of 8.6μg/mL.Thus,according to the GC/MS analysis,the vasodilatory activity of perilla oil was relevant to the monoterpenoids.And perilla aldehyde was found to exhibit certain vasodilatory activity.The perilla oil was found to showed vasodilatory activity for the first time.And further study showed that perilla aldehyde was an active constituent,which provided a new basis for the research and development of the perilla leaves.

关 键 词：紫苏叶 挥发油 舒张血管 紫苏醛 

分 类 号：R285[医药卫生—中药学]