基于空气动力辅助解吸电喷雾质谱技术的指纹化学成像研究  

Chemical Analysis and Imaging of Fingerprints by Air-flow Assisted Desorption Electrospray Ionization Mass Spectrometry

作　　者：史俊稳[1,2] 郑令娜[2] 马荣梁[3] 汪冰[2] 陈汉清[2] 王萌[2] 王海芳[1] 丰伟悦[2] SHI Jun-Wen;ZHENG Ling-Na;MA Rong-Liang;WANG Bing;CHEN Han-Qing;WANG Meng;WANG Hai-Fang;FENG Wei-Yue(School of Environmental and Chemical Engineering,Shanghai University,Shanghai 200444,China;CAS Key Laboratory for Biomedical Effects of Nanomaterials and Nanosafety,Institute of High Energy Physics,Chinese Academy of Sciences,Beijing 100049,China;Institute of Forensic Science,Ministry of Public Security,Beijing 100038,China)

机构地区：[1]上海大学环境与化学工程学院,上海200444 [2]中国科学院高能物理研究所,中科院纳米生物效应与安全性重点实验室,北京100049 [3]公安部物证鉴定中心,北京100038

出　　处：《分析化学》2019年第12期1909-1914,共6页Chinese Journal of Analytical Chemistry

基　　金：国家自然科学基金项目(Nos.11505194,11875268);国家重点研发计划项目(No.2016YFA0201604);痕迹科学与技术公安部重点实验室开放课题(No.2017FMKFKT07)资助~~

摘　　要：指纹分析在法庭科学科研和实践中具有重要意义。与现有的指纹显影方法相比,基于质谱的成像方法不仅可识别检测指纹中的痕量化学成分,还可获得清晰高分辨的指纹形态。本研究采用空气动力辅助解吸电喷雾质谱成像(AFADESI-MSI)技术,在45 L/min的空气流辅助下,以5μL/min乙腈为解吸喷雾溶剂,喷雾电压7000 V,正离子模式(100~1000 Da)下,分析了按压在打印纸上的汗液、印泥、防晒霜、粉底液共4种指纹。结果表明,采用AFADESI-MSI技术不仅能得到指纹中多种内源性和外源性物质的化学信息,还能得到指纹的高分辨图像。此外,根据指纹中的化学信息能够区分叠加的指纹。AFADESI-MSI技术作为一种新的指纹分析方法,有望在法庭科学研究和司法鉴定中得到更广泛的应用。Fingerprint analysis is of great significance in forensic sciences. Compared with existing fingerprint analytical methods, mass spectrometry-based methods can not only identify trace chemical components in fingerprints, but also obtain fingerprint imaging. In this study, four kinds of fingerprints, including sweat, inkpad, sunscreen, and liquid foundation, were analyzed by air-flow assisted desorption electrospray ionization mass spectrometry imaging(AFADESI-MSI). AFADESI was employed with the air flow of 45 L/min and 5 μL/min acetonitrile was used as spray solvent at spray voltage 7000 V. Positive ion full scan mode(100-1000 Da) was chosen. The results showed that AFADESI-MSI technology could not only obtain chemical information of various endogenous and exogenous substances in fingerprints, but also obtain high resolution images of fingerprints. In addition, overlapped fingerprints could be distinguished according to the typical chemical information in fingerprints. As a new fingerprint analysis method, AFADESI-MSI will be widely used in forensic scientific research and practical applications.

关 键 词：空气动力辅助解吸电喷雾质谱 指纹分析 化学成像 

分 类 号：TP3[自动化与计算机技术—计算机科学与技术]