新型比色荧光双通道探针用于硫化氢的检测  

A Novel Colorimetric and Fluorescent Probe for Detection of Hydrogen Sulfide

作　　者：王肖莉 姚猛 李引 魏超[1] 王美 WANG Xiao-Li;YAO Meng;LI Yin;WEI Chao;WANG Mei(College of Chemistry and Environmental Science,Hebei University,Baoding 071002,China;The Flame Retardant Material and Processing Technology Engineering Research Center of Hebei Province,Baoding 071002,China)

机构地区：[1]河北大学化学与环境科学学院,保定071002 [2]河北省阻燃材料与加工技术工程技术研究中心,保定071002

出　　处：《分析化学》2019年第12期1915-1921,共7页Chinese Journal of Analytical Chemistry

基　　金：河北省自然科学基金项目(Nos.B2019201418,B2018201234);河北省高等学校科学技术研究项目(No.QN2017015);保定市科学研究与发展计划(No.18ZF315)资助~~

摘　　要：以7-硝基苯并呋咱(NBD)为荧光团,经一步有机合成,高产率制备比色荧光双通道探针,用于检测环境和体内硫化氢(H2S)的含量。以无水Na2S为H2S供体,考察了探针的选择性、灵敏性等性能。结果表明,探针荧光强度与H2S浓度在5~50μmol/L浓度范围内呈良好的线性关系,检出限为0.125μmol/L,同时反应溶液由无色变为紫色。采用MTT法测试探针的细胞毒性,结果表明,在探针浓度低于50μmol/L条件下,细胞存活率高于90%。激光扫描共聚焦显微成像结果表明,探针可靶向细胞溶酶体,可对溶酶体内H2S进行成像研究。To detect the level of hydrogen sulfide(H2S) in the environment and living beings, a colorimetric and fluorescent turn-off probe based on selective thiolyling of NBD(7-nitro-1,2,3-benzoxadiazole) amino was prepared through one-step organic synthesis. The selectivity and sensitivity of the probe were investigated by using sodium sulfide as an H2S donor in PBS buffer. The fluorescent intensity of the probe exhibited good linear relationship with concentration of H2S in the concentration range of 5-50 μmol/L, and the detection limit was estimated to be 0.125 μmol/L. The color of the solution changed from light yellow to light violet. The cell survival rate was higher than 90% when the concentration of the probe was below 50 μmol/L. The results of cell imaging experiments showed that the probe could be used for imaging H2S in the lysosomes of the cells.

关 键 词：硫化氢 比色检测 荧光探针 细胞成像 

分 类 号：R28[医药卫生—中药学]