应用FISH-AFLP技术分析105份樱桃种质资源的亲缘关系  

Genetic Relationship Analysis of 105 Accessions of Cherry Germplasms by FISH-AFLP Technology

作　　者：陈新[1] 徐丽[1] 宗晓娟[1] 王甲威[1] 谭钺[1] 朱东姿 魏海蓉[1] 洪坡 刘庆忠[1] Chen Xin;Xu Li;Zong Xiaojuan;Wang Jiawei;Tan Yue;Zhu Dongzi;Wei Hairong;Hong Po;Liu Qingzhong(Shandong Institute of Pomology/Shandong Provincial Key Laboratory of Fruit Tree Biotechnology Breeding,Taian 271000,China)

机构地区：[1]山东省果树研究所/山东省果树生物技术育种重点实验室

出　　处：《山东农业科学》2019年第11期22-27,共6页Shandong Agricultural Sciences

基　　金：山东省农业科学院青年科研基金项目(2016YQN25);山东省现代农业产业技术体系果品创新团队项目(SDAIT-06-04);科技部科技基础平台项目(NICGR2018-048)

摘　　要：为建立樱桃种质资源的FISH-AFLP技术体系并进行亲缘关系分析,本研究以国内外搜集的105份樱桃种质为对象,经DNA提取、EcoRⅠ/MseⅠ双酶切,再进行FISH-AFLP反应。扩增结果显示,筛选得到8对EcoRⅠ/MseⅠ多态性引物,可扩增出1148条谱带,平均多态性比率98.61%。聚类分析和遗传多样性分析结果显示,105份樱桃栽培品种的遗传相似性系数为0.54~0.89,当阈值为0.68时,可分成7个AFLP群;有效等位基因数、基因多样度、Shannon信息指数分别为1.52、0.30、0.45,具有较高的遗传多样性。本研究结果可为今后樱桃品种鉴别和遗传多样性研究等提供理论指导。The objective of this study was to establish FISH-AFLP analysis system for cherry and analyze the genetic relationship of the main cultivars.One hundred and five cherry cultivars were selected as experiment materials.The high-quality genomic DNA was extracted and the purified DNA samples were digested with EcoRⅠ/MseⅠ;after ligation reaction,the samples were used to perform FISH-AFLP detection.The amplification results showed that eight pairs of EcoRⅠ/MseⅠpolymorphic primers were screened and 1148 bands were obtained.The average percentage of polymorphic bands was 98.61%.The results of cluster analysis and genetic diversity analysis showed that the genetic similarity coefficient of the 105 cherry cultivars varied from 0.54 to 0.89.All the samples were divided into 7 groups when the threshold value was 0.68.The numbers of effective alleles,gene diversity and Shannon information index of the main cultivars were 1.52,0.30 and 0.45,respectively,indicating high genetic diversity.This study could provide theoretical guidance for the identification and genetic diversity research of cherry varieties in the future.

关 键 词：樱桃 FISH-AFLP 遗传多样性 亲缘关系 

分 类 号：S662.5[农业科学—果树学]