miR-146a-5p对人牙髓干细胞成牙本质分化的影响  

The effects of miR-146a-5p on the odontogenic differentiation potential of human dental pulp stem cells

作　　者：邱在玲 林诗晗 曾建钗 柯志红 肖婷婷 吕红兵[1] QIU Zailing;LIN Shihan;ZENG Jianchai;KE Zhihong;XIAO Tingting;LV Hongbing(Depart-ment of Endodontics,School and Hospital of Stomatology,Fujian Medical University,Fuzhou 350002,China)

机构地区：[1]福建医科大学附属口腔医院牙体牙髓科

出　　处：《实用口腔医学杂志》2019年第6期830-833,共4页Journal of Practical Stomatology

基　　金：福建省自然科学基金面上项目(编号:2018J01817);福建省卫生计生科研人才培养项目(编号:2018-1-64)

摘　　要：目的:探讨miR-146a-5p对人牙髓干细胞成牙本质分化的调控作用。方法:前期通过二代测序技术检测人牙髓干细胞和根尖牙乳头干细胞中miRNAs的差异表达,用qRT-PCR验证前期筛选出的7个miRNAs的表达水平。通过重组慢病毒转染人牙髓干细胞,特异性上调miR-146a-5p后进行成牙本质诱导,用茜素红染色,碱性磷酸酶活性检测和qRT-PCR的方法检测成牙本质分化能力的改变。结果:miR-146a-5p在人牙髓干细胞中的表达显著高于根尖牙乳头干细胞。过表达miR-146a-5p后矿化结节、ALP活性和成牙本质分化标志基因表达增加。结论:miR-146a-5p促进人牙髓干细胞向成牙本质分化。Objective: To investigate the effects of miR-146 a-5 p in regulating odontogenic differentiation of human dental pulp stem cells(DPSCs). Methods: In the previous study, miRNAs expression of DPSCs and stem cells from the apical papilla(SCAPs) were screened by the Next generation sequencing, 7 differentialy expressed miRNAs were identified by qRT-PCR. DPSCs were stably transfected by miR-146 a-5 p overexpression lentivirus and treated continuously with odontogenic induction medium. Alizarin red staining, alkaline phosphatase(ALP) activity and the expression levels of odontogenic marker genes were analyzed after transfection. Results: miR-146 a-5 p expression was significantly upregulated in DPSCs compared with SCAPs. The mineralized nodules, ALP activity and the expression levels of odontogenic marker genes increased obviously in miR-146 a-5 p overexpression group compared with the control group. Conclusion: miR-146 a-5 p can promote the odontogenic differentiation of DPSCs.

关 键 词：牙髓干细胞(DPSCs) 根尖牙乳头干细胞(SCAPs) MIRNAS 成牙本质分化 

分 类 号：R329.2[医药卫生—人体解剖和组织胚胎学] R781.3[医药卫生—基础医学]