青藏高原东缘高原鼢鼠种群抗药性评估  

Anticoagulant resistance evaluation in plateau zokor(Eospalax baileyi) based on Vkorc1 gene variation in eastern Qinghai-Tibetan Plateau

作　　者：谭宇尘 韩天虎 许国成 魏彦明[3] 蔡志远 王缠 姚宝辉 郭怀亮 苏军虎[4] TAN Yuchen;HAN Tianhu;XU Guocheng;WEI Yanming;CAI Zhiyuan;WANG Chan;YAO Baohui;GUO Huailiang;SU Junhu(College of life Science and Technology,Gansu Agricultural University,Lanzhou 730020,Gansu,China;Grassland Technology Extension Station of Gansu Province,Lanzhou 730010,Gansu,China;College of veterinary medicine,Gansu Agricultural University,Lanzhou 730020,Gansu,China;College of Grassland Science,Key Laboratory of Grassland Ecosystem(Ministry of Education),Pratacultural Engineering Laboratory of Gansu Province,Sino-U.S.Centers for Grazing land Ecosystem Sustainability,Gansu Agricultural University,Lanzhou 730070,Gansu,China)

机构地区：[1]甘肃农业大学生命科学技术学院,甘肃兰州730070 [2]甘肃省草原技术推广总站,甘肃兰州730020 [3]甘肃农业大学动物医学院,甘肃兰州730070 [4]甘肃农业大学草业学院,草业生态系统教育部重点实验室,中美草地畜牧业可持续发展研究中心,甘肃兰州730070

出　　处：《草业科学》2019年第11期2952-2961,共10页Pratacultural Science

基　　金：甘南草原主要害鼠的抗药性监测及其治理策略(GCB2015-01);甘肃省陇原青年创新创业人才项目“不育剂对高原鼢鼠种群的控制作用及其可持续机制”(LQ20180916-2);甘肃农业大学“伏羲杰出人才”培育项目(Gaufx-02J03)

摘　　要：啮齿动物对抗凝血剂类药物的抗性通常与维生素K环氧化物还原酶复合物亚单位1(Vkorc1)基因变异有关。本研究检测了青藏高原东缘8个地区高原鼢鼠(Eospalax baileyi)Vkorc1基因3个外显子的核苷酸变异情况,测序鉴别其基因型,并结合已知突变分析其抗药性。结果发现,在PCR结果检测中成功获得高原鼢鼠Vkorc1基因3个外显子区,并检测出多态性的存在。870只高原鼢鼠个体中,有197只个体的Vkorc1氨基酸序列与其野生型有差异。地理分布上,有5个种群的Vkorc1基因检测出了突变,共界定了4个基因型。高原鼢鼠Vkorc1氨基酸存在4个错义突变位点,其中在4个高原鼢鼠种群中检测到了Phe63Cys氨基酸突变与抗药性相关的基因型。对比褐家鼠(Rattus norvegicus)氨基酸序列,发现高原鼢鼠Vkorc1基因有18个错义突变位点。研究为青藏高原东缘高原鼢鼠抗性(地理)种群的管理及其抗药性的治理提供了依据。Rodent resistance to coagulant drugs is usually associated with vitamin K epoxide reductase complex subunit 1(Vkorc1)genetic variation.In this study,we analyzed nucleotide variation of three exons of the Vkorc1 gene in the plateau zokor(Eospalax baileyi)in eight regions of the eastern margin of the Qinghai-Tibet Plateau,through sequencing to identify genotypes and analysis of drug resistance in association with known mutations.For results,the three exons of the Vkorc1 gene of the plateau zokor were successfully obtained through PCR,and the presence of polymorphism was detected.Among the 870 plateau zokor individuals,197 individuals had different Vkorc1 amino acid sequences from the wild type sequence.Geographically,Vkorc1 gene mutations were detected in five populations,and four genotypes were defined.Four missense mutation sites in Vkorc1 amino acid of plateau zokors were detected.Phe63 Cys amino acid mutations were detected in four plateau zokor populations.When comparing the amino acid sequences with Rattus norvegicus,18 missense mutations were found in the Vkorc1 gene of plateau zokor.This study provides a basis for the geographic management of resistant zokor populations and the control of resistance in the eastern margin of the Qinghai Tibetan plateau.

关 键 词：高原鼢鼠 抗凝血剂类药物 抗药性 Vkorc1基因 

分 类 号：S443.2[农业科学—农业昆虫与害虫防治]