一种基于甲基纤维素半固体培养基的真菌单孢分离新方法  

A novel method to isolate fungal single-spores using semisolid methylcellulose medium

作　　者：秦鹏[1] 路等学[1] 康红梅[1] 韩融冰[1] 郭瑞[1] 赵玉卉[1] 魏甲乾[1] 王治业[1,2] QIN Peng;LU Dengxue;KANG Hongmei;HAN Rongbing;GUO Rui;ZHAO Yuhui;WEI Jiaqian;WANG Zhiye(Institute of Biology,Gansu Academy of Sciences,Lanzhou 730000,China;Key Laboratory of Microbial Resources Exploitation and Application of Gansu Province,Institute of Biology,Gansu Academy of Sciences,Lanzhou 730000,China)

机构地区：[1]甘肃省科学院生物研究所,甘肃兰州730000 [2]甘肃省科学院生物研究所,甘肃省微生物资源开发利用重点实验室,甘肃兰州730000

出　　处：《食品与发酵工业》2019年第21期79-85,共7页Food and Fermentation Industries

基　　金：甘肃省青年科技基金计划资助(17JR5RA181);甘肃省科学院应用研究与开发项目(2017JK-14);甘肃省科学院合作项目(2014HZ-01);甘肃省科学院应用研究与开发项目(2018JK-06);甘肃省科学院应用研究与开发项目(2017JK-07);国家自然科学基金地区科学基金计划(31560037)

摘　　要：利用优化的甲基纤维素培养基与传统的平板涂布、显微操作、有限稀释和琼脂糖培养基方法,对蛹虫草菌孢子悬液(理论活孢子数量为150个)进行单孢分离,比较可分离单菌落数量、操作简易度及分离效率等参数,分析甲基纤维素半固体培养基分离真菌单孢的可行性。结果表明,甲基纤维素的优化质量浓度为23 g/L。甲基纤维素培养基、平板涂布、显微操作、有限稀释和琼脂糖培养基方法依次获得可分离单菌落96、70、37、35和26个,甲基纤维素培养基方法的操作最简单、便捷和高效。优化的甲基纤维素半固体培养基可有效分离真菌单孢,简便易行,应用潜力优于传统方法。Taking Cordyceps militaris as an illustrative example,a novel method that opened up an optional way for the isolation of fungal single-spores based on semisolid methylcellulose medium was developed.Optimized methylcellulose medium and traditional methods(spread plate,micromanipulation,limited dilution,and agarose medium)were used to isolate single-spores of Cordyceps militaris spore suspension that theoretically contained 150 living spores.Comparing the number of partible single colonies,operation simplicity and the efficiency of single-spore isolation,the feasibility of this novel method to isolate fungal single-spores was investigated.The optimized concentration of methylcellulose in semisolid medium was 23 g/L.The number of partible single colonies obtained using the optimized methylcellulose medium,spread plate,micromanipulation,limited dilution and optimized agarose medium reached 96、70、37、35 and 26,respectively.This novel method was proved to be the simplest,most convenient and efficient among these methods.Fungal single-spores could be simply,efficiently and conveniently isolated using optimized methylcellulose medium.The applied potential of the novel method is superior to that of traditional methods.

关 键 词：甲基纤维素 单孢分离 蛹虫草菌 

分 类 号：TS2[轻工技术与工程—食品科学与工程]