免疫途径对绵羊支原体肺炎灭活疫苗安全性和抗体水平的影响  

Effect of vaccination routes on the safety and antibody level of a Mycoplasma ovipneumoniae bacterin

作　　者：袁婷 吴娅琴 郝华芳[2] 陈胜利[2] 颜新敏[2] 史耀旭 赵天荣[2] 储岳峰[2] 孙延鸣[1] YUAN Ting;WU Ya-qin;HAO Hua-fang;CHEN Sheng-li;YAN Xin-min;SHI Yao-xu;ZHAO Tian-rong;CHU Yue-feng;SUN Yan-ming(College of Animal Science and Technology,Shihezi University,Shihezi 832001,China;State Key Laboratory of Veterinary Etiological Biology/Lanzhou Veterinary Research Institude,Chinese Academy of Agricultural Science,Lanzhou 730046,China;Shandong Taifeng Biological Products Incorporation Limited,Jinan,250306,China)

机构地区：[1]石河子大学动物科技学院,新疆石河子832001 [2]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室,甘肃兰州730046 [3]山东泰丰生物制品有限公司,山东济南250306

出　　处：《中国兽医科学》2019年第11期1422-1426,共5页Chinese Veterinary Science

基　　金：国家重点研发计划项目（2017YFD0500905）;家畜疫病病原生物学国家重点实验室重大成果培育项目（2016-7）;国家科技支撑计划项目（2015BAD12B02）

摘　　要：为了评价免疫途径对绵羊支原体肺炎灭活疫苗安全性和免疫效果的影响,将该疫苗分别于颈部皮下和肌肉各接种205只绵羊,对全身性反应和注射局部进行安全性观察,并于免疫当天和免疫后第21天各采集50只绵羊血分离血清,用绵羊肺炎支原体ELISA试剂盒检测血清抗体阳转率和抗体水平。结果,皮下注射组(19.5%)与肌肉注射组(17.6%)全身性不良反应均较高,差异不显著;但皮下注射组(36.6%)的局部不良反应显著高于肌肉注射组(2.4%);肌肉注射组抗体阳转率(89.8%)和平均D450值升高水平(免疫后比免疫前升高0.89)均显著高于皮下注射组(77.1%,0.45)。上述结果表明,肌肉注射绵羊支原体肺炎灭活疫苗的安全性和免疫效果优于皮下注射。To evaluate the effect of inoculation routes on the safety and immune efficacy of a Mycoplasma ovipneumoniae bacterin,a group of 205 sheep were inoculated with the bacterin subcutaneously and another group of 205 sheep were performed intramuscularly,respectively.Systemic adverse reactions(SAR)and local adverse reactions(LAR)of injection sites were monitored and recorded,meanwhile the antibody levels of 100 sheep were determined by a M.ovipneumoniae specific ELISA assay and seroconversion rates on day 21 post-infection(dpi)were calculated.The results showed that 19.5%of subcutaneous group had SAR,and so did 17.6%of intramuscular group.For the numbers of sheep which showed LAR,the subcutaneous group(36.6%)were significantly more than the intramuscular group(2.4%).The seroconversion rates(89.8%)and antibody level changes(the difference of D450 value is 0.89)of the intramuscular group are both significantly higher than the subcutaneous group(77.1%,D450 value difference is 0.49).In conclusion,sheep vaccinated intramuscularly with the Mycoplasma ovipneumoniae bacterin is safer and more efficient than those vaccinated subcutaneously.

关 键 词：绵羊支原体肺炎灭活疫苗 肌肉注射 皮下注射 安全性 抗体水平 

分 类 号：S852.62[农业科学—基础兽医学]