利用ISSR标记鉴定双孢蘑菇的传统菌株类型  

Identification of Agaricus bisporus traditional strain types by using ISSR markers

作　　者：施肖堃[1] 郭仲杰[1] 蔡志欣[1] 卢园萍 陈美元[1] 廖剑华[1] Shi Xiaokun;Guo Zhongjie;Cai Zhixin;Lu Yuanping;Chen Meiyuan;Liao Jianhua(The Edible Fungi Institute of Fujian Academy of Agricultural Sciences,National and Local Joint Engineering Research Center for Breeding&Cultivation of Featured Edible Fungi,Fuzhou 350014,Fujian,P.R.China)

机构地区：[1]福建省农业科学院食用菌研究所特色食用菌繁育与栽培国家地方联合工程研究中心

出　　处：《食药用菌》2019年第6期399-403,共5页Edible and Medicinal Mushrooms

基　　金：福建省省属公益类科研院所基本科研专项(2019R1035-2,2018R1020-5);现代农业产业技术体系专项资金(CARS20);福建省种业创新与产业化工程项目(fjzycxny2017009);福建省农业科学院科技创新团队(STIT2017-1-6)

摘　　要：具有优质或高产性状的双孢蘑菇传统菌株是品种改良的重要亲本来源。通过对双孢蘑菇具有代表性的24个优质类型和24个高产类型传统菌株的ISSR-PCR分析,发现引物ISSR834、ISSR835、ISSR807共扩增产生7条能判别传统菌株优质类型或高产类型的特异性标记条带,可以在双孢蘑菇定向杂交育种中方便地区分和鉴定传统菌株亲本的优质或高产性状。Agaricus bisporus traditional strains with good-quality or high-production are important parent resource for strain improvement. By using ISSR-PCR analysis of representative 24 good-quality and 24 high-production traditional strains of A. bisporus, it was found that the primer ISSR834, ISSR835 and ISSR807 produce 7 special bands for distinguishing traditional strain types, and they could be used in the identification of good-quality or high-production characteristics of traditional strain parent in the directional crossbreeding of A. bisporus.

关 键 词：双孢蘑菇 菌株类型 ISSR 特异条带 DNA标记 

分 类 号：S646.11[农业科学—蔬菜学]