艾灸对胃荷瘤大鼠瘤体内Th1、Th2类细胞因子表达的影响  

Effects of moxibustion on the expression of Th1 and Th2 cytokines in gastric tumor-bearing rats

作　　者：谭静[1] 林亚平[1] 赵欢 彭卓隽 欧阳里知 陈欲攀 石俊林 TAN Jing;LIN Yaping;ZHAO Huan;PENG Zhuojun;OUYANG Lizhi;CHEN Yupan;SHI Junlin(School of Acupuncture and Tuina,Hunan University of Chinese Medicine,Changsha 410208,China)

机构地区：[1]湖南中医药大学针灸推拿学院

出　　处：《免疫学杂志》2019年第12期1061-1066,共6页Immunological Journal

基　　金：国家自然科学基金（81574077）;湖南省教育厅优秀青年项目（18B257)

摘　　要：目的观察艾灸对胃荷瘤大鼠瘤体内Th1、Th2类细胞因子表达的影响。方法 40只体质量200~240 g SPF级SD大鼠适应性喂养1周后,采用手术胃部移植Walker-256瘤组织建立胃荷瘤模型。7 d后随机选取10只验证造模成功,剩余30只随机分入模型组、艾灸组及红外组,每组10只。自入组当日起,艾灸组第1日悬灸中脘、关元、双侧足三里,第2日悬灸双侧脾俞及胃俞;红外组第1日红外线照射胃脘部,第2日照射背部T12-T13棘突间区域;模型组第1日仰卧位固定,第2日俯卧位固定。各组每次干预20 min,每日1次,连续21 d。干预期间密切观察动物摄食量、体质量,对生存状态进行积分。干预结束后,处死动物,测量胃部瘤体体积,测算生长抑制率。利用细胞因子微阵列芯片对瘤体中Th1、Th2类细胞因子进行筛查,ELISA法测定其含量。结果干预结束后,与模型组比较,艾灸组动物生存状态改善,摄食量及体质量增加(均P<0.01),胃部瘤体增长受限(P<0.01),抑制率达41.89%,瘤体内Th1类细胞因子TNF-α、INF-γ含量增加(P<0.01,P<0.05),Th2类细胞因子IL-6、IL-22含量减少(P<0.01,P<0.05);与模型组比较,红外组动物生存状态、体质量稍改善(均P<0.05),胃部瘤体增长稍受限,抑制率为28.09%,瘤体内TNF-α含量增加(P<0.05),IL-6含量减少(P<0.01);与红外组比较,艾灸组动物摄食量、生存状态积分改善更明显(P<0.01,P<0.05),胃部瘤体体积更小(P<0.05),瘤体内INF-γ含量更高(P<0.05)。结论艾灸及红外治疗能不同程度地改善荷瘤动物的生存状态,抑制瘤体生长,其作用可能与增加瘤体内Th1类细胞因子TNF-α、INF-γ,减少Th2类细胞因子IL-6、IL-22有关。To observe the effect of moxibustion on the expression of Th1/Th2 cytokines in gastric tumorbearing rats, total of 40 SD rats(200~240 g) were recruited in this study. After one week’s adaptive feeding,Walker-256 tumor tissues were transplanted onto the stomach of the SD rats to establish the gastric tumor-bearing model. Seven days later, 10 rats were randomly selected to verify the success of the model. The remaining 30 rats were randomly divided into model group, moxibustion group and infrared group(10 rats per group). The rats in moxibustion group were received moxibustion with Zhongwan(CV 12), Guanyuan(CV 4), Zusanli(ST 36) at 1 stday and moxibustion with Pishu(BL 20), Weishu(BL 21) at the 2 ndday;the rats in infrared group were irradiated with infrared ray on epigastric region at 1 stday and irradiated the T12-T13 interspinous process area on the back at the2 ndday;the rats in model group were fixed in supine position at 1 stday and prone position at 2 ndday. The intervention of each group was last 20 min per time, one time per day for 21 days. During the treatment, the survival status, food intake and body weight were recorded. After treatment, the rats were sacrificed to measured the tumor volume on the stomach and calculate the tumor growth inhibition rate. Furthermore, tumor-related cytokines were detected by protein microarray chip, while retested Th1 and Th2 cytokines level of the tumor tissues were detected by ELISA.Compared with rats in model group, rats in moxibustion group demonstrated higher levels of survival rate, food intake, and body quality(all P<0.01);the stomach tumor of moxibustion group was limited(P<0.01) and the growth inhibition rate was 41.89%;the levels of Th1 cytokines TNF-α and INF-γ in gastric tumors increased significantly(P<0.01, P<0.05), while Th2 cytokines IL-6 and IL-22 decreased significantly(P<0.01, P<0.05);compared with the model group, the food intake and survival status of the animals in the infrared group were improved(P<0.01, P<0.05), the growth of tumor volume in t

关 键 词：艾灸 胃癌 微阵列芯片 TH1细胞因子 TH2细胞因子 

分 类 号：R392.12[医药卫生—免疫学]