籽用南瓜细菌性果腐病种子带菌检测与消毒处理  

Detection of Bacterial Fruit Blotch in Seed-used Pumpkin and Seed Disinfection

作　　者：田金丽 王曼曼 王云莉 屈淑平 徐文龙 崔崇士 TIAN Jinli;WANG Manman;WANG Yunli;QU Shuping;XU Wenlong;CUI Chongshi(College of Horticulture and Landscape rchitecture,Northeast Agricultural University,Harbin,Heilongjiang 150030)

机构地区：[1]东北农业大学园艺园林学院

出　　处：《北方园艺》2019年第22期60-64,共5页Northern Horticulture

基　　金：公益性行业科研专项资助项目(201303112)

摘　　要：以市场销售的5份籽用南瓜种子为试材,采用PCR检测技术对南瓜种子细菌性果腐病菌进行检测,用不同浓度的CuSO4、HCl、H2O2、过氧乙酸和漂白粉等药剂处理带菌种子进行消毒处理。研究了不同药剂处理对南瓜种子果腐病菌消毒效果的影响。结果表明:市场销售的5份南瓜种子均携带细菌性果腐病菌;5种药剂对南瓜种子携带的细菌性果腐病菌均有消毒效果,但HCl、H2O2、过氧乙酸对南瓜种子的发芽有抑制作用。1.0%CuSO4处理30 min和1.5%漂白粉处理30 min对南瓜种子果腐病菌防治效果最好,并且2种药剂处理对种子发芽率和发芽势影响不明显。因此,选用1.0%CuSO4或1.5%漂白粉对南瓜种子进行消毒处理,可以消除种子表皮所携带的果腐病病菌。Seeds of(Cucurbita maxima)from market were detected for bacterial fruit blotch(BFB)by polymerase chain reaction(PCR)technology.To select the best treatment for seed disinfection,CuSO4,HCl,H2O2,peroxyacetic acid and bleaching powder with different concentrations treated the seeds-borne BFB.The effect of different agentia treatment on the disinfection of pumpkin seed rot pathogen was studied.The results showed that:BFB was detected in 5 different kind of seeds from market.In our study,all 5 treatments with different concentrations had bactericidal effect.However,HCL,H2O2 and peroxyacetic acid also had inhibitory effects on the germination of pumpkin seeds.1.0%CuSO4 with 30 minutes treatment and 1.5%bleaching powder with 30 minutes treatment achieved the best effect on BFB inhibition,and had no effect on seed germination.So,after 1.0%CuSO4 with 30 minutes treatment or 1.5%bleaching powder with 30 minutes treatment,BFB carried by pumpkin seeds could be effectively inhibited.

关 键 词：南瓜 药剂 果腐病 防治效果 抑菌 

分 类 号：S642.1[农业科学—蔬菜学]