清溃愈疡汤对溃疡性结肠炎肠道菌群、炎症应激及相关因子水平的影响  

Effect of the Qingkui Yuyang Decoction on intestinal flora, inflammatory stress, and related factors in ulcerative colitis

作　　者：单琳琳[1] 陆为民 张卿[1] SHAN Lin-lin;LU Wei-min;ZHANG Qing(Gastroenterology,Lianyungang Hospital Affiliated with Nanjing University of Traditional Chinese Medicine,Lianyungang,Jiangsu,China222000;Gener l Medicine,Nanjing University of Traditional Chinese Medicine Hospital,N njing,Jiangsu,China210029)

机构地区：[1]南京中医药大学连云港附属医院消化内科,江苏连云港222000 [2]南京中医药大学附属医院普内科,江苏南京210029

出　　处：《中国病原生物学杂志》2019年第10期1211-1214,共4页Journal of Pathogen Biology

基　　金：连云港市卫生科技项目(No.201512)

摘　　要：目的探讨清溃愈疡汤对溃疡性结肠炎肠道菌群、炎症应激及相关因子水平的影响。方法选择2016年2月-2018年1月本院收治的溃疡性结肠炎患者120例,采用抽签法分为常规治疗组(60例)和观察组(60例)。常规治疗组给予双岐杆菌三联活菌肠溶胶囊联合美沙拉嗪肠溶片治疗,观察组在常规治疗的基础上给予清溃愈疡汤灌肠治疗,比较两组患者肠道菌群、炎症应激及相关因子水平的变化。结果治疗前,两组患者比较肠道菌群分布数量、炎症应激及相关因子水平差异均无统计学意义(P>0.05)。治疗后,两组患者肠球菌、肠杆菌数量, MCP-1、MIP-1α、HMGB1、HIF-1α、TGF-β1及β-EP水平较治疗前均显著降低(均P<0.01),双歧杆菌、乳酸杆菌及丁酸梭菌的数量显著增多(均P<0.01);观察组患者肠球菌、肠杆菌数量及MCP-1、MIP-1α、HMGB1、HIF-1α、TGF-β1、β-EP水平分别为(7.11±0.71)log10CFU/g、(8.22±0.91)log10CFU/g、(45.63±4.71)ng/mL、(10.65±1.28)ng/mL、(21.15±2.25)pg/mL、(49.11±5.09)ng/L、(128.47±14.06)ng/L及(5.54±0.67)pg/100μL,与常规治疗组比较差异均有统计学意义(t值分别为8.672、7.261、9.235、20.386和22.926、5.338、8.513和37.388,均P<0.01)。双歧杆菌、乳酸杆菌及丁酸梭菌数量与常规治疗组比较差异均无统计学意义(均P>0.05)。结论清溃愈疡汤方剂可显著改善溃疡性结肠炎患者的肠道菌群紊乱状态,缓解炎症应激,抑制HIF-1α、TGF-β1和β-EP的过表达,进而保护肠黏膜的屏障功能,促进患者的恢复。Objectives To investigate the effects of the Qingkui Yuyang Decoction on intestinal flora, inflammatory stress, and related factors in ulcerative colitis. Methods Subjects were a total of 120 patients with ulcerative colitis seen at this Hospital from February 2016 to January 2018. Patients were randomly divided into a conventional treatment group(n=60) and an observation group(n=60). The conventional treatment group was given bifidobacterium triple live enteric-coated capsules combined with mesalazine enteric-coated tablets. The observation group was given the Qingkui Yuyang Decoction in addition to the conventional treatment. The changes in intestinal flora, inflammatory stress, and related factors were compared between the two groups. Results There were no significant differences in the distribution and amount of intestinal flora and levels of inflammatory stress and related factors between the two groups compared to measurements before treatment(P>0.05). After treatment, the amount of enterococci and enterobacteria and levels of the inflammatory markers MCP-1, MIP-1á, HMGB1, HIF-1á, TGF- 1, and -EP decreased significantly in the two groups, while the amount of bifidobacteria, lactobacilli, and Clostridium butyricum increased significantly(P<0.01 for all). The amount of enterococci and enterobacteria in the observation group were 7.11±0.71 log10 CFU/g 8.22±0.91 log10 CFU/g and levels of MCP-1, MIP-1á, HMGB1, HIF-1á, TGF- 1, and -EP were 45.63±4.71 ng/mL, 10.65±1.28 ng/mL, 21.15±2.25 pg/mL, 49.11±5.09 ng/L, 128.47±14.06 ng/L, and 5.54±0.67 pg/100ìL. The amount of enterococci and enterobacteria and levels of inflammatory markers differed significantly from those in the conventional treatment group(P<0.01 for all)(t values = 8.672, 7.261, 9.235, 20.386 and 22.926, 5.338, 8.513 and 37.388, P<0.01 for all). There were no significant differences in the amount of bifidobacteria, lactobacilli, and Clostridium butyricum compared to amounts in the conventional treatment group(P>0.05). Conclusion The Qingkui

关 键 词：清溃愈疡汤 溃疡性结肠炎 肠道菌群 炎症因子 

分 类 号：R378[医药卫生—病原生物学]