雷帕霉素雾化治疗小鼠气道变应性炎症的探索研究  

Exploratory study of rapamycin atomization in the treatment of airway allergic inflammation in mice

作　　者：李峥科 韦屹[1] 卢通 孙伟[1] 文卫平[1] LI Zhengke;WEI Yi;LU Tong;SUN Wei;WEN Weiping(Department of Otolaryngology Head and Neck Surgery,the First Affiliated Hospital of Sun Yat-sen University,Guangzhou,Guangdong,510080,China)

机构地区：[1]中山大学附属第一医院耳鼻咽喉头颈外科

出　　处：《中国耳鼻咽喉头颈外科》2019年第10期561-566,共6页Chinese Archives of Otolaryngology-Head and Neck Surgery

基　　金：国家自然科学基金资助项目(K0113097)

摘　　要：目的探讨雷帕霉素雾化吸入在治疗小鼠气道变应性炎症中的作用。方法 30只SPF级雌性Balb/c小鼠随机分成5组,每组6只,分别为对照组、气道变应性炎症模型组、气道变应性炎症模型+雷帕霉素10 mg/kg组、气道变应性炎症模型+雷帕霉素15 mg/kg组和气道变应性炎症模型+雷帕霉素20 mg/kg组。建模完成后记录小鼠挠鼻和打喷嚏次数,分别用HE染色和PAS染色评估肺部炎症,ELISA法检测肺泡灌洗液、鼻腔灌洗液炎症因子以及血清卵清蛋白(ovalbumin,OVA)特异性IgE浓度。结果与对照组相比较,模型组小鼠挠鼻和打喷嚏次数显著增加,肺组织炎症程度显著增加,肺泡灌洗液和鼻腔灌洗液Th2炎症因子IL-4、IL-5及IL-13浓度显著增加,血清OVA特异性IgE浓度增加。和模型组相比较,雷帕霉素处理组小鼠挠鼻和打喷嚏次数显著下降,肺组织炎症程度降低,肺泡灌洗液和鼻腔灌洗液Th2炎症因子IL-4、IL-5及IL-13显著下降,血清OVA特异性IgE浓度增加。结论雷帕霉素雾化治疗可以缓解小鼠气道变应性炎症,雷帕霉素雾化治疗有望成为控制和治疗气道变应性炎症的新方法。OBJECTIVE To explore the effect of rapamycin atomization inhalation on the treatment of airway allergic inflammation in mice. METHODS 30 SPF female Balb/c mice aged from 6 to 8 weeks were randomly and equally divided into 5 groups(n=6). Named as follows:Control group, airway allergic inflammation model group, airway allergic inflammation+rapamycin 10 mg/kg group, airway allergic inflammation+rapamycin 15 mg/kg group, and airway allergic inflammation+rapamycin 20 mg/kg group. The frequency of nasal scratching and sneezing were recorded after the airway allergic inflammation model established, HE staining and PAS staining were used to evaluate the lung inflammation. ELISA was used to detecting the concentration of cytokines in bronchoalveolar lavage fluid(BALF), nasal lavage fluid(NALF) and the concentration of serum ovalbumin(OVA) specific Ig E. RESULTS Compared with the control group, the frequency of sneezing and nasal scratching in model group mice were markedly aggravated. The inflammation levels of lungs were markedly aggravated in model group mice. The concentration of Th2 inflammation cytokines IL-4, IL-5 and IL-13 in BALF and NALF increased significantly, and the concentration of serum OVA-specific Ig E was markedly aggravated. Compared with the model group, the frequency of nasal scratching and sneezing in rapamycin treatment group mice were markedly aggravated, the inflammation levels of lungs were markedly alleviated in rapamycin treated group mice,and the concentration of Th2 inflammation cytokines IL-4, IL-5 and IL-13 in BALF and NALF were decreased significantly, and the concentration of serum OVA-specific Ig E were markedly alleviated. CONCLUSION Rapamycin atomization inhalation is able to alleviate airway allergic inflammation in mice. Rapamycin atomization inhalation maybe considered as a new therapeutic method in treatment of airway allergic inflammation.

关 键 词：投药 吸入 TH2细胞 西罗莫司 雷帕霉素 气道变应性炎症 卵清蛋白 

分 类 号：R28[医药卫生—中药学]