钆贝葡胺增强磁共振成像对大鼠肝纤维化的诊断价值  

Diagnostic value of gadobenate dimeglumine enhanced MRI in hepatic fibrosis of rats

作　　者：余蕊 倪晓琼 亚洋 范国华[1] Yu Rui;Ni Xiaoqiong;Ya Yang;Fan Guohua(Department of Radiology,the Second Affiliated Hospital of Soochow University,Suzhou 215004,China)

机构地区：[1]苏州大学附属第二医院影像科,苏州215004

出　　处：《中华医学杂志》2019年第43期3428-3431,共4页National Medical Journal of China

基　　金：苏州市科技计划项目:民生科技-关键技术应用研究(SS201854)。

摘　　要：目的探讨钆贝葡胺(Gd-BOPTA)增强磁共振成像对大鼠肝纤维化分期的诊断价值.方法50只雄性SD大鼠(6周龄,体质量180~220 g)分为建模组(n=42)和对照组(n=8),建模组采用四氯化碳法建立肝纤维化模型,动物许可证编号SYXK(苏)2017-0043.实验第2周至第10周每周随机抽取建模组(n=4)和对照组(n=1)大鼠于注射Gd-BOPTA后1 h(RER1)、2 h(RER2)、3 h(RER3)分别扫描,测算肝实质的相对强化率(RER).观察肝内胆管形态及各时间点强化程度.结果42只大鼠(建模组34只、对照组8只)完成实验.对照组RER1、RER2、RER3分别为1.44±0.37、1.22±0.37、0.84±0.28.建模组RER1、RER2、RER3分别为:S1期(n=6):1.49±0.48、1.29±0.39、0.91±0.38;S2期(n=9):1.48±0.44、1.34±0.37、1.04±0.40;S3期(n=11):1.49±0.43、1.37±0.39、1.21±0.30;S4期(n=8):1.49±0.44、1.40±0.37、1.24±0.40.对照组与肝纤维化组RER1、RER2值比较差异均无统计学意义(F=0.022,P=0.999;F=0.301,P=0.875);对照组与肝纤维化S3期、S4期RER3值比较差异有统计学意义(t=2.249,P=0.031;t=2.274,P=0.029),其余各组间比较差异无统计学意义(均P>0.05).对照组增强后1 h肝内胆管明显强化,走行自然;肝纤维化S3-S4期增强后1 h肝内胆管轻度强化,2~3 h肝内胆管明显强化,走行扭曲.结论Gd-BOPTA增强后延迟3 h肝实质相对强化率及肝内胆管扭曲延迟强化有助于肝纤维化S3-S4期诊断.Objective To evaluate the value of Gd-BOPTA enhanced MRI in the staging of liver fibrosis.Method Fifty male SD rats(6-week-old,180-220 g)were divided into the modeling group(n=42)and the control group(n=8).The model of liver fibrosis in the modeling group was established by carbon tetrachloride(animal license No.SYXK(Su)2017-0043).From week 2 to week 10,rats in the modeling group(n=4)and control group(n=1)were randomly selected to scan 1 h(RER1),2 h(RER2)and 3 h(RER3)after injection of Gd-BOPTA,respectively,to measure the relative enhancement rate(RER)of liver parenchyma.The shape of intrahepatic bile duct and the degree of enhancement at each time point were observed.Results Forty-two rats(34 rats in the modeling group and 8 rats in the control group)completed the experiment.RER1,RER2 and RER3 of the control group were 1.44±0.37,1.22±0.37 and 0.84±0.28 respectively.RER1,RER2 and RER3 of the modeling group were respectively:S1(n=6):1.49±0.48,1.29±0.39,0.91±0.38;S2(n=9):1.48±0.44,1.34±0.37,1.04±0.40;S3(n=11):1.49±0.43,1.37±0.39,1.21±0.30;S4(n=8):1.49±0.44,1.40±0.37,1.24±0.40.There was no significant difference in RER1 and RER2 values between the control group and the liver fibrosis group(F=0.022,P=0.999;F=0.301,P=0.875).There were significant differences between the control group and RER3 values of hepatic fibrosis stage S3 and S4(t=2.249,P=0.031;t=2.274,P=0.029),there was no significant difference between the remaining groups(all P>0.05).In the control group,the intrahepatic bile duct was obviously strengthened within 1 hour after enhancement,and walked naturally.The intrahepatic bile duct was slightly enhanced 1h after the enhancement of S3-S4 stage of hepatic fibrosis,and the intrahepatic bile duct was significantly enhanced 2-3 hours later,with distorted alignment.Conclusion Delayed 3 hours liver parenchymal RER and intrahepatic bile duct distortion delay enhancement after Gd-BOPTA enhancement contribute to the S3-S4 diagnosis of liver fibrosis.

关 键 词：肝硬化 钆 磁共振成像 大鼠 诊断 

分 类 号：R65[医药卫生—外科学]