喷他佐辛对慢性缩窄性损伤模型大鼠皮肤P物质及神经激肽1受体表达的影响  

Effects of pentazocine on substance P and neurokinin-1 receptors skin expression in chronic constriction injury rat

作　　者：毛豪丽[1] 刘春芳[1] 方舒东[1] 支延康[1] MAO Hao-li;LIU Chun-fang;FANG Shu-dong;ZHI Yan-kang(Department of Anesthesiology,Shanghai Ninth People's Hospital,Shanghai JiaoTong University School of Medicine,SHA NGHAI 200011,China)

机构地区：[1]上海交通大学医学院附属第九人民医院麻醉科

出　　处：《中国新药与临床杂志》2019年第11期677-680,共4页Chinese Journal of New Drugs and Clinical Remedies

摘　　要：目的观察喷他佐辛对坐骨神经慢性缩窄性损伤(CCI)模型大鼠疼痛阈值、皮肤中P物质(SP)及神经激肽1受体(NK1-R)表达的影响。方法 36只雄性SD大鼠随机分为对照组、模型组和治疗组,每组12只。暴露模型组和治疗组大鼠坐骨神经给予三线点扎,观测得造模后第15日达疼痛高峰期。治疗组于造模后第15日单次给予喷他佐辛10 mg·kg^-1股部肌内注射,其他2组不给药。观察各组大鼠疼痛阈值变化,采用免疫组化法和RT-PCR法检测大鼠皮肤中SP及NK1-R蛋白和mRNA的表达水平。结果与造模前比较,造模后第15日模型组和治疗组疼痛阈值均显著降低(P <0.05),且均低于对照组(P <0.05)。治疗组大鼠治疗后疼痛阈值显著升高(P <0.05),且高于模型组(P <0.05)。模型组SP及NK1-R蛋白和mRNA的表达水平显著高于对照组(P <0.05),治疗组SP及NK1-R蛋白和mRNA的表达水平显著低于模型组(P<0.05)。结论喷他佐辛对CCI模型大鼠坐骨神经慢性疼痛有明显的镇痛效果,其机制可能与降低皮肤中SP及NK1-R的表达有关。AIM To explore the effects of pentazocine on pain threshold and expression of substance P( SP) and neurokinin-1 receptors( NK1-R) in skin of ischiadic nerve chronic constriction injury( CCI)model rats. METHODS Thirty-six male SD rats were randomly divided into 3 groups and 12 rats each. The ischiadic nerve of rats in model group and treatment group were ligatured by three knots. After 15 days, the pain threshold reached high peak. The rats in the treatment group were treated with a single dose of pentazocine10 mg·kg^-1 intramuscular injection at 15 th day after modeling. The rats in the model group and control group were untreated. The pain threshold were observed and the expression of SP and NK1-R protein and mRNA in skin were examined by immunohistochemistry and RT-PCR. RESULTS Compared with before the model establishment, the pain thresholds in the model group and treatment group were significantly lower(P < 0.05)on the 15 th day after modeling, and were lower than the control group(P < 0.05). The pain threshold of the treatment group was significantly increased after treatment(P < 0.05), and higher than the model group(P <0.05). The expression levels of SP and NK1-R protein and mRNA in the model group were significantly higher than those in the control group(P < 0.05). The expression levels of SP and NK1-R protein and mRNA in the treatment group were significantly lower than those in the model group(P < 0.05). CONCLUSION Pentazocine has obvious analgesic effect on chronic pain of sciatic nerve in CCI model rats, and its mechanism may be related to reducing the expression of SP and NK1-R in skin.

关 键 词：喷他佐辛 镇痛 P物质 神经激肽1受体 慢性缩窄性损伤 

分 类 号：R971[医药卫生—药品]