3种桑叶黑茶的饮用安全性评价  

Drinking Safety Evaluation of 3 Kinds of Mulberry Leaf Dark Tea

作　　者：邵元元 颜新培 李飞鸣 肖建中 邹湘月 李霞 叶添梅 Shao Yuanyuan;Yan Xinpei;Li Feiming;Xiao Jianzhong;Zou Xiangyue;Li Xia;Ye Tianmei(Sericultural Research Institute of Hunan Province,Changsha 410127,China;Key Laboratory of Tea Science,Ministry of Education,Hunan Agricultural University,Changsha 410128,China;Hunan Co-Innovation Center for Utilization of Botanical Functional Ingredients,Changsha 410128,China)

机构地区：[1]湖南省蚕桑科学研究所,长沙410127 [2]湖南农业大学茶学教育部重点实验室,长沙410128 [3]湖南省植物功能成分利用协同创新中心,长沙410128

出　　处：《蚕业科学》2019年第4期555-562,共8页ACTA SERICOLOGICA SINICA

基　　金：湖南省重点研发计划课题(No.2015NK3054);现代农业产业技术体系建设专项(No.CARS-18)

摘　　要：以桑叶茯砖、桑叶千两和桑叶天尖为研究对象,采用食品安全国家标准测定方法对3种桑叶黑茶中致病菌、重金属和农药残留进行检测,并按食品安全毒理评价程序对桑叶黑茶进行急性毒性试验,以期探明桑叶黑茶的饮用安全性。检测结果显示,桑叶茯砖、千两、天尖3种黑茶样品中不含致病菌,重金属铅、镉、汞和砷的含量都远低于茶叶农业标准,同时均未检测出农药残留,符合国家标准对食品中污染物限量的要求。小鼠急性毒性试验结果显示,桑叶茯砖、千两、天尖提取物对小鼠的LD50分别为22.3 g/kg、22.8 g/kg、28.5 g/kg,均大于《保健食品评价技术与规范》中规定的无毒值;灌胃3种黑茶提取物的小鼠脏器解剖未发现明显异常改变。研究结果表明,3种桑叶黑茶属于无毒级食品,符合食品安全要求。Taking 3 kinds of mulberry leaf dark tea Fuzhuan, Qianliang and Tianjian as research objects, their residues of pathogenic bacteria, heavy metals and pesticide were detected in accordance to the national food safety standards, and acute toxicity test was performed in line with the food safety toxicological evaluation procedure to ensure the drinking safety of mulberry leaf dark tea. The results showed that there was no pathogenic bacteria in the samples of mulberry leaf dark tea Fuzhuan, Qianliang and Tianjian. Moreover, content of heavy metals such as lead, cadmium, mercury and arsenic was far below agricultural standards for tea, and no pesticide residue was detected, which met the national stan-dards for contaminant limits in food. The test on acute toxicity to mice indicated that LD50 of mulberry leaf dark tea Fuzhuan, Qianliang and Tianjian were 22.3 g/kg, 22.8 g/kg and 28.5 g/kg, respectively, all of which were higher than non-toxic standard defined in Health Food Evaluation Technology and Specification. Besides, no significant abnormal changes were found in the viscera of mice intragastrically administrated with mulberry leaf dark tea extracts. All above results proved that mulberry leaf dark tea is non-toxic food and meets the requirements of food safety.

关 键 词：桑叶黑茶 安全性评价 致病菌 重金属 农药残留 致死中量 

分 类 号：S886.9[农业科学—特种经济动物饲养] TS272.5[农业科学—畜牧兽医]