生姜中黄曲霉毒素液质联用高通量检测研究  

Study on a high-throughput method with LC-MS/MS technique for detecting aflatoxins in ginger

作　　者：张莹[1] 刘文超[1] 杨艳丽[1] 舒志强 李浪 Zhang Ying;Liu Wenchao;Yang Yanli;Shu Zhiqiang;Li Lang(Sun Simiao School of Medicine,Tongchuan Vocational and Technical College,Tongchuan 727031,China;Shaanxi origin Agricultural Technology Co.,Ltd.,Tongchuan 727031,China)

机构地区：[1]铜川职业技术学院孙思邈医学院,727031 [2]陕西起源农业科技有限责任公司,铜川727031

出　　处：《国际中医中药杂志》2019年第11期1236-1240,共5页International Journal of Traditional Chinese Medicine

摘　　要：目的建立超高效液相色谱-串联质谱法(ultra high performance liquid chromatography-tandem mass spectrometry,UPLC-MS/MS)同时测定生姜中黄曲霉毒素B1、B2、G1、G2含量。方法采用甲醇-水(80∶20,V/V)提取生姜,氮气吹干浓缩;采用Waters Acquity UPLC BEH C18色谱柱(2.1 mm×50 mm,1.7μm),流动相为0.1%甲酸-水(A相)-0.1%甲酸-甲醇(B相),梯度洗脱,流速0.35 ml/min;质谱采用电喷雾离子源,正离子扫描模式,多反应离子监测。结果4种黄曲霉毒素采用基质匹配标准曲线进行定量,在0.125~20.000 ng/ml范围内线性关系良好,相关系数均大于0.9990。样品检出限在0.125~0.300μg/kg之间,定量限在0.125~1.000μg/kg之间,平均加标回收率为81.7%~96.0%,RSD均小于7.53%。结论所建立的UPLC-MS/MS方法简单、快速、灵敏,检出限低,可用于生姜中痕量黄曲霉毒素残留的测定。Objective To optimize the method of simultaneous determination of four aflatoxins(B1,B2,G1 and G2)of ginger by the ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)method and high-throughput method.Methods The aflatoxins were extracted from ginger by methanol-water(80:20,V/V)solution,concentrated and dried with nitrogen.The aflatoxins were detected by UPLC-MS/MS by using Waters Acquity UPLC BEH C18 chromatographic column.The mobile phase was 0.1%formic acid water(A phase)-0.1%formic acid methanol(B phase),gradient elution,flow rate 0.35 ml/min,mass spectrometry was electrospray ion source,positive ion scanning mode,multi reaction ion monitoring were using.Results Quantification of four aflatoxins by matrix matching standard curve.The linear was good in the range of 0.125-20.000 ng/ml,and the correlation coefficients were all greater than 0.9990.The ginger sample detection was 0.125-0.300μg/kg and 0.125-1.000μg/kg,respectively.The average recoveries were 81.7%-96.0%,and the relative standard deviation(RSD)was lower than 7.53%.Conclusions This method is simple,rapid,sensitive and low limit of detection,which can meet the requirements for the detection of trace aflatoxins residues in ginger.

关 键 词：生姜 串联质谱法 黄曲霉毒素类 高通量检测 

分 类 号：R28[医药卫生—中药学]