紫花草总黄酮对于大鼠草酸钙结石模型的作用及机制研究  

Study on the effect and mechanism of total flavonoids from Mosla for rats with calcium oxalate stone in kidney

作　　者：胡建军 童方运 何军 Hu Jianjun;Tong Fangyun;He Jun(Department of Urology,Hunan Childrens Hospital,Changsha 410007 China)

机构地区：[1]湖南省儿童医院(南华大学儿科学院)泌尿外科,长沙410007

出　　处：《国际泌尿系统杂志》2019年第6期1031-1034,共4页International Journal of Urology and Nephrology

摘　　要：目的 探讨紫花草总黄酮对草酸钙结石模型SD雄性大鼠的治疗效果及机制,为草酸钙结石的防治提供思路.方法 采用1%乙二醇+1%氯化铵经典大鼠草酸钙结石为模型.分别以安慰剂、总黄酮低剂量、总黄酮高剂量治疗.测大鼠24 h尿液中草酸的含量、浓度,测定肾组织及尿液中钙离子、镁离子的含量;测血清中尿素氮(BUN)、肌酐( Cr)及血清钙离子的含量,观察肾脏病理变化及草酸钙结晶并评分.结果 不同剂量紫草花总黄酮治疗大鼠肾脏组织钙离子含量、24 h尿钙离子量、肾组织的草酸钙晶体及肾小管扩张均低于模型组( P<0. 05).结论 紫草花总黄酮能够有效防止SD雄性大鼠肾脏草酸钙结石形成.Objective To explore the effect and mechanism of total flavonoids on calcium oxalate stones in SD male rats,and to provide ideas for the prevention and treatment of calcium oxalate stones.Methods The model of 1%ammonium chloride+1%ethylene glycol was used.The patients were treated with placebo,low dose of total flavones and high dose of total flavones.The content and concentration of oxalic acid in rats'24-hour urine were measured.The contents of calcium and magnesium in urine were determined,and the levels of blood urea,creatinine and serum calcium were determined,HE staining was performed on the kidney sections of rats,and the pathological changes and calcium oxalate crystals were observed and graded under 100-fokl microscope.Results The contents of calcium ions in renal tissues,24-hour urinary calcium ions,calcium oxalate crystals in renal tissues and renal tubular dilatation in rats treated with different doses of flavonoids from comfreys flower were significantly lower than those in the model group(P<0.05).Conclusions The total flavonoids could prevent the formation of calcium oxalate in kidney of SD male rats.

关 键 词：尿路结石 草酸钙 紫苜蓿 黄酮 

分 类 号：R69[医药卫生—泌尿科学]