通过KEGG生物通路富集分析探析人参皂苷Rh1对乳腺癌SKBR3细胞基因表达的影响  

Analysis of the Effect of Ginsenoside Rh1 on Gene Expression of Breast Cancer SKBR3 Cells by KEGG Biological Pathway Enrichment Analysis

作　　者：李秋华[1] 鞠伶伟 王宁[1] 刘兆喆 LI Qiu-hua;JU Ling-wei;WANG Ning;LIU Zhao-zhe(The Second Affiliated Hospital of Liaoning University of Traditional Chinese Medicine,Shenyang Liaoning 110034,China;Northern Theater General Hospital,Shenyang Liaoning 110016,China)

机构地区：[1]辽宁中医药大学附属第二医院,辽宁沈阳110034 [2]北部战区总医院,辽宁沈阳110016

出　　处：《中医药导报》2019年第20期36-41,共6页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：2017年辽宁省中医药临床专(学)科能力建设项目;2018年辽宁省博士启动基金

摘　　要：目的:基于前期筛查人参皂苷Rh1干预下乳腺癌SKBR3细胞中差异表达的基因,对差异表达基因的功能及相关信号通路进行分析。方法:应用生物信息学对差异表达基因的功能及相关信号通路进行分析。结果:KEGG生物通路富集分析发现差异表达基因在人类乳头瘤病毒感染、剪接体、基底黏附、细胞凋亡、mTOR信号通路、MAPK信号通路、TNF信号通路、泛素-蛋白酶体通路等信号通路中富集显著。结论:人参皂苷Rh1可能通过调控核糖核蛋白复合物的生物合成、基底黏附、泛素-蛋白酶体等通路抑制SKBR3细胞的活性。Objective: To analyze the function of differentially expressed genes and related signaling pathways, based on the screen-out differentially expressed genes in breast cancer SKBR3 cells under the intervention of ginsenoside Rh1. Methods: Bioinformatics was used to analyze the function and related signaling pathways of differentially expressed genes. Results: KEGG enrichment analysis showed that the differentially expressed genes were significantly different in the signaling pathways of HPV infection, splice, basal adhesion,apoptosis, mTOR signaling pathway MAPK, TNF and ubiquitin-proteasome pathway signaling pathways. Conclusion: Ginsenoside Rh1 may inhibit the activity of SKBR3 cells by regulating the biosynthesis of ribonucleoprotein complexes, basal adhesion, and ubiquitin-proteasome pathways.

关 键 词：乳腺癌 SKBR3细胞 人参皂苷RH1 KEGG生物通路 基因表达 

分 类 号：R273[医药卫生—中西医结合]