响应曲面法优选毛头牛蒡子中总木脂素类成分的提取工艺研究  

Optimization of Extraction Process of Total Lignans from Maotou Niubangzi(Arctium Tomentosum Mill) by Response Surface Methodology

作　　者：刘富铭 杨明翰 付晓[1] 葛亮[1] LIU Fu-ming;YANG Ming-han;FU Xiao;GE Liang(Xinjiang Medical University Institute of Traditional Chinese Medicine/Xinjiang Autonomous Region Experimental Teaching Demonstration Center of Traditional Chinese Medicine,Urumqi Xinjiang 830011,China)

机构地区：[1]新疆医科大学中医学院/新疆自治区级中医药学实验教学示范中心

出　　处：《中医药导报》2019年第20期48-51,59,共5页Guiding Journal of Traditional Chinese Medicine and Pharmacy

基　　金：新疆地产中药民族药新药研发培育项目(Q2015-02-07)

摘　　要：目的:采用响应曲面法对毛头牛蒡子中总木脂素的提取工艺进行优化。方法:在单因素试验的基础上,以加醇倍量、乙醇浓度和提取时间3个因素为自变量,以总木脂素含量、牛蒡苷含量、浸膏得率的综合评分为因变量,运用响应曲面法进行优化。结果:总木脂素的最佳提取工艺为加醇倍量为14.7倍,乙醇浓度为58%,提取2次,每次62 min。结论:响应曲面法优选毛头牛蒡子总木脂素的提取工艺方法简单、稳定、可行。Objective: To optimize the extraction process of total lignans from Maotou Niubangzi(arctium tomentosum Mill) by response surface methodology. Methods: On the basis of single factor experiment, the three factors of alcohol addition, ethanol concentration and extraction time were used as independent variables, and the comprehensive scores of total lignan content, arctiin content and extract yield were used as dependent variables.The response surface methodology was used for predictive analysis. Results: The optimal extraction process of total lignans was 14.7 times the alcohol addition, 58% ethanol concentration, 2 extractions, and 62 minutes each time. Conclusion: Response surface methodology is a simple, stable and feasible method for optimizing the extraction process of total lignans from Maotou Niubangzi(arctium tomentosum Mill).

关 键 词：毛头牛蒡子 总木脂素 响应曲面法 提取工艺 

分 类 号：R284[医药卫生—中药学]