肾积水模型小鼠肾组织中EphrinA1、血管内皮生长因子、miRNA-210的表达  

Expression of Ephrin A1,vascular endothelial growth factor and microRNA-210 after hydronephrosis

作　　者：雍江[1] 何军[1] 彭潜龙[1] Yong Jiang;He Jun;Peng Qianlong(Department of Urology Hunan Children s Hospital,Changsha 410007,China)

机构地区：[1]湖南省儿童医院小儿泌尿外科

出　　处：《中国医师杂志》2019年第11期1672-1675,共4页Journal of Chinese Physician

摘　　要：目的研究肾积水(UUO)模型小鼠肾组织中Ephrin A1、血管内皮生长因子(VEGF)、miRNA-210表达的差异性及相关性,了解三者在小鼠肾组织损伤发生的调控机制。方法40只小鼠随机分为急性肾积水组(UUO组)和假手术对照组(NC组),其中UUO组随机分为四个亚组(UUO2 d组、UUO 5 d组、UUO 9 d组和UUO 14 d组),五组各8只小鼠。UUO组采用单侧输尿管结扎建立小鼠肾积水模型。HE染色观察肾组织病理形态学变化;实时荧光定量PCR(qRT-PCR)检测Ephrin A1、VEGF及miRNA-210的表达;Western blot检测Ephrin A1和VEGF蛋白的表达。结果NC组未显示肾积水,UUO组显示小鼠肾积水及炎症细胞浸润。与NC组相比,UUO 2、5 d组小鼠Ephrin A1mRNA、VEGF mRNA及miRNA-210的表达上调(P<0.05);随着肾积水时间的延长,9、14 d组Ephrin A1 mRNA、VEGF mRNA及miRNA-210的表达较2、5 d组均下调,在14 d时达到最低点(P<0.05);Western blot检测发现,与NC组相比,UUO 2、5 d组小鼠肾组织Ephrin A1、VEGF蛋白表达增加(P<0.05)。结论UUO小鼠模型验证了miRNA-210参与了肾积水过程,Ephrin A1、VEGF、miRNA-210表达的一致性说明了三者在UUO的发生与发展过程中发挥重要作用。Objective To investigate the difference and correlations of the Ephrin A1,vascular endothelial growth factor(VEGF)expression and microRNA(miRNA)-210 in the kidney tissue of rats in the unilateral ureteral obstruction(UUO)model.And to understand the regulation mechanism of the three factors in the occurrence of renal tissue injury in rats.Methods Using unilateral ureteral ligation to establish a model of hydronephrosis in mice(except for the NC group),and they wrere randomly divided into an acute hydronephrosis group(UUO group)and a false surgical control group(NC group).The UUO group was divided into four groups(UUO 2 d group,UUO 5 d group,UUO 9 d group,and UUO 14 d group),and eight mice in each group.The pathological changes of renal tissue were observed by hematoxylin-eosin(HE)staining.Quantitative real-time polymerase chain reaction(qRT-PCR)was used to detect the mRNA expression of Ephrin A1,VEGF and miRNA-210;Western blot was used to detect the protein expression of Ephrin A1 and VEGF.Resulsts Hydronephrosis was not shown in the NC group,and hydronephrosis and inflammatory cell infiltration were observed in the UUO group.Compared with NC group,the mRNA expression of Ephrin A1,VEGF and miRNA-210 were up-regulated in UUO 2 d group(P<0.05).With the extension of the time of hydronephrosis,the expressions of all three factors were down-regulated in 9 d and 14 d groups compared with 2 d and 5 d groups,and reached the lowest point at 14 d(P<0.05);Western blot result indicated that the expression of Ephrin A1 and VEGF in renal tissue of UUO 2 d and 5 d group was increased compared with NC group(P<0.05).Conclusions The UUO mouse model verified that miRNR-210 participated in hydronephrosis.The consistency of Ephrin A1,VEGF,and miRNA-210 indicated that the three factors played an important role in the occurrence and development of UUO.

关 键 词：肾盂积水 膜附着型配体A1 血管内皮生长因子 微RNAS 小鼠 

分 类 号：R69[医药卫生—泌尿科学]