口服人参皂苷Rg1提高鸡的肠道黏膜免疫  

Oral administration of ginsenoside Rg1 enhances gut mucosal immunity in chickens

作　　者：毕师诚[1] 马晓丹[1] 吴烨 崔雪梅 胡松华[1] BI Shi-cheng;MA Xiao-dan;WU Ye;CUI Xue-mei;HU Song-hua(College of Animal Sciences,Zhejiang University,Hangzhou 310058 China)

机构地区：[1]浙江大学动物科学学院

出　　处：《中国兽医学报》2019年第11期2215-2221,共7页Chinese Journal of Veterinary Science

基　　金：国家重点研发计划资助项目(2017YFD0502200)

摘　　要：将96只SPF母鸡随机分成4组,1,4组注射生理盐水;2,3组连续3d肌肉注射环磷酰胺(Cy)诱导免疫抑制。3组在Cy引起免疫抑制后连续7d每天饮水口服1mg/kg人参皂甙Rg1,其余组只饮水。1,2,3组于给药结束后免疫传染性法氏囊病毒(IBDV)疫苗,4组不免疫。于给药前和免疫后1,2,3周计算脾脏和法氏囊指数,采血测定抗体阳性率,检测十二指肠灌洗液总sIgA和特异性sIgA含量;观察十二指肠黏膜上皮内淋巴细胞(IELs)以及IgA+细胞数。于免疫后1周提取十二指肠总RNA,检测肠组织免疫相关基因mRNA的表达。结果表明,和免疫抑制组相比,饮水口服1mg/kg人参皂苷Rg1可提高IBDV抗体阳性率,显著增加脾脏和法氏囊指数,显著提高肠道总sIgA和特异性sIgA含量;此外,口服Rg1显著增加了十二指肠IELs和固有层IgA+细胞数量,上调了鸡十二指肠TLR4、p65、TGF-β、pIgR和CCR9基因的mRNA表达水平。Ninety-six chickens were randomly divided into 4 groups,each consisting of 24 birds.Groups 2 and 3 received intramuscular injection of cyclophosphamide(Cy) at 100 mg/kg body weight for 3 d to induce oxidative stress and immune suppression.Groups 1 and 4 were injected with saline in the same way as groups 2 and 3.Then chickens in group 3 were orally administrated Rg1 of 1 mg/kg body weight in drinking water for 7 d.After that,groups 1 to 3 were orally vaccinated with attenuated infectious bursal disease(IBDV) vaccine.Relative weights of spleen and bursa of Fabricius were measured before administration and 1,2,3 weeks after immunization.Serum was obtained to investigate positive rate of antibody.Duodenal lavage fluid was collected to detect total sIgA and specific sIgA content.Duodenal tissue was collected to observe the number of intraepithelial lymphocytes(IELs) and IgA+ cells in the lamina propria.The total RNA of duodenum was extracted first week after immunization,and the expression of mRNA was detected by RT-qPCR.The results showed that oral administration of 1 mg/kg ginsenoside Rg1 could significantly increase the positive rate of antibody,spleen and bursa index and intestinal total sIgA and specific sIgA content compared with the Cy group.In addition,oral administration of Rg1 significantly increased the number of iIELs and IgA+ cells in the lamina propria of the duodenum,as well as up-regulated the mRNA expression of TLR4,p65,TGF-beta,pIgR and CCR9 genes in the chicken duodenum.

关 键 词：人参皂甙RG1 环磷酰胺 黏膜免疫 

分 类 号：S853.7[农业科学—临床兽医学]