HPLC测定民族药滇白珠抗炎镇痛活性部位(ARF)中4种活性成分的含量  

Quantitative Determination of Four Active Constituents in Anti-inflammatory and Analgesic Active Fraction of Gaultheria leucocarpa var. yunnanensis

作　　者：李晓 王秀环 赵怡程[1] 王乐 范书生 王小萍 许啸 闫昕[1] 何婷[1] 常艳丽[1] 折改梅[1] LI Xiao;WANG Xiu-huan;ZHAO Yi-cheng;WANG Le;FAN Shu-sheng;WANG Xiao-ping;XU Xiao;YAN Xin;HE Ting;CHANG Yan-li;SHE Gai-mei(School of Chinese Materia Medica,Beijing University of Chinese Medicine,Beijing 102488,China)

机构地区：[1]北京中医药大学中药学院

出　　处：《中国实验方剂学杂志》2019年第24期91-96,共6页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81573692)

摘　　要：目的:建立高效液相色谱法(HPLC)测定民族药滇白珠抗炎镇痛活性部位(ARF)中4种活性成分methyl benzoate-2-O-β-D-xylopyranosyl(1-2)[O-β-D-xylopyranosyl(1-6)]-O-β-D-glucopyranoside(MSTG-A),methyl benzoate-2-O-β-Dglucopyranosyl(1-2)[O-β-D-xylopyranosyl(1-6)]-O-β-D-glucopyranoside(MSTG-B),gaultherin及绿原酸的含量测定方法,为滇白珠药材的深入研究及其质量控制提供方法依据,并为后期滇白珠的制剂与临床应用奠定基础。方法:采用COSMOSIL 5C18-PAQ色谱柱(4.6 mm×250 mm,5μm),以甲醇-0.2%冰乙酸水溶液为流动相梯度洗脱,柱温25℃,流速1 m L·min^-1,检测波长294 nm。结果:MSTG-A,MSTG-B,gaultherin,绿原酸分别在0.01406~0.45000,0.00781~0.25000,0.00313~0.10000,0.00094~0.03000 g·L^-1线性关系良好(r≥0.9997);方法精密度、重复性、稳定性良好;平均加样回收率分别在100.81%,99.19%,96.12%,102.56%,其RSD分别为1.4%,0.7%,0.7%,2.4%。滇白珠ARF部位中MSTG-B,MSTG-A,gaultherin,绿原酸质量分数分别为23.608,41.973,8.282,2.673 mg·g^-1。结论:该方法操作简便、准确、重复性好,适用于滇白珠抗炎镇痛活性部位中4种活性成分的含量测定,为滇白珠药材的深入研究、质量控制与综合评价提供参考依据,并为后期滇白珠药材的制剂与临床应用奠定基础。Objective:To establish a high performance liquid chromatography(HPLC)method for determination of four active constituents,namely MSTG-A,MSTG-B,gaultherin and chlorogenic acid in the antiinflammatory and analgesic active fraction(ARF)of the ethnic medicine Gaultheria leucocarpa var.yunnanensis,in order to provide a methodological basis for the in-depth study and quality control of G.leucocarpa var.yunnanensis,and lay a foundation for later preparation and clinical application.Method:The determination was performed on COSMOSIL 5 C18-PAQ(4.6 mm×250 mm,5μm)column with methanol-0.2%glacial acetic acid(gradient elution)as the mobile phase at a flow rate of 1 m L·min^-1.The column temperature was 25℃.The detection wavelength was set at 294 nm.Result:The linear range of MSTG-B,MSTG-A,gaultherin and chlorogenic acid were 0.01406-0.45000,0.00781-0.25000,0.00313-0.10000,0.00094-0.03000 g·L^-1(r≥0.9997),respectively,with a good precision,repeatability and stability.And the average recoveries were100.81%,98.99%,96.12%and 102.56%,respectively.RSDs were 1.4%,0.7%,0.7%,2.4%,respectively.The contents of MSTG-B,MSTG-A,gaultherin and chlorogenic acid in ARF fraction of G.leucocarpa var.yunnanensis were 23.608,41.973,8.282,2.673 mg·g^-1,respectively.Conclusion:The established method was simple and accurate,with a high repeatability.It can be used for determination of four active constituents in ARF fraction of G.leucocarpa var.yunnanensis,so as to provide a reference for the in-depth research,quality control and comprehensive evaluation of G.leucocarpa var.yunnanensis and lay a solid foundation for preparation and clinical application.

关 键 词：滇白珠 活性部位 MSTG-A MSTG-B gaultherin 绿原酸 含量测定 

分 类 号：R284.2[医药卫生—中药学] R289[医药卫生—中医学]