水稻OsERF103基因生物信息学分析、亚细胞定位及表达分析  

Bioinformatics Analysis, Subcellular Localization and Expression Analysis of OsERF103 Gene in Rice(Oryza sativa)

作　　者：邹杰[1] 李生强[1] 刘显军[1] 陈刚 ZOU Jie;LI Sheng-Qiang;LIU Xian-Jun;CHEN Gang(College of Life Science and Environmental Resources/Jiangxi Provincial Key Laboratory of Crop Growth and Development Regulation,Yichun University,Yichun 336000,China)

机构地区：[1]宜春学院生命科学与资源环境学院/江西省作物生长发育调控重点实验室

出　　处：《农业生物技术学报》2019年第12期2130-2139,共10页Journal of Agricultural Biotechnology

基　　金：国家自然科学基金项目(No.31460061);江西省自然科学基金项目(No.20141521080006)

摘　　要：乙烯应答因子(ethylene responsive factors,ERFs)是一类植物特有的转录因子,在植物的生长发育和逆境胁迫响应中起重要调控作用。为了研究水稻(Oryza sativa)OsERF103基因(Gen Bank No.XM015768788)的功能,本研究利用生物信息学方法对其序列特征进行分析;构建了p BWA(V)HS-OsERF103-Glosgfp融合表达载体并转化水稻原生质体,通过激光共聚焦显微镜观察融合蛋白在水稻原生质体的亚细胞定位;利用q RT-PCR技术对该基因在水稻不同组织及不同逆境胁迫下的表达模式进行分析。结果表明OsERF103含有1个AP2(APETALA2)结构域,为亲水性蛋白,不含信号肽和跨膜结构,在进化关系上与短舌野生稻(Oryza brachyantha)类脱落酸阻遏因子1(abscisic acid repressor 1-like,ABR1-like)蛋白的亲缘关系最近;亚细胞定位结果显示OsERF103定位于细胞核;q RT-PCR分析结果显示,OsERF103在孕穗期的根、茎、叶、叶鞘、叶枕和幼穗中均有表达,其在根中表达最强,在叶和幼穗中表达较弱;OsERF103被高温、低温、PEG6000、高盐和脱落酸(abscisic acid,ABA)诱导表达,但在不同的胁迫条件下该基因表达模式不尽相同。本研究为进一步研究水稻OsERF103基因的功能提供了基础资料。Ethylene responsive factors(ERFs)are plant-specific transcription factors that play important roles in plant developmental process and in response to abiotic stress.In order to explore the function of OsERF103(Gen Bank No.XM015768788)in rice(Oryza sativa),bioinformatics methods were used to analyze the sequence characteristic of OsERF103.For subcellular localization analysis,the fusion expression vector of p BWA(V)HS-OsERF103-Glosgfp was constructed and transformed into rice protoplast,then laser scanning confocal microscopy was used to observe the subcellular localization of OsERF103 fusion protein in rice protoplast;Expression analysis of OsERF103 in different tissues and under different abiotic stress conditions was conducted by q RT-PCR.The results showed that OsERF103 was a hydrophilic and unstable protein without signal peptide and transmembrane structure,and contained an AP2(APETALA2)domain.Phylogenetic analysis revealed the OsERF103 was evolutionarily closest to a abscisic acid repressor 1-like(ABR1-like)protein of Oryza brachyantha.The results of subcellular localization showed that OsERF103 was located in the nucleus.OsERF103 transcripts were detected in all sampled tissues at booting stage,with the highest level detected in root and weak level detected in leaf and young spike.OsERF103 transcripts were induced by high temperature,low temperature,PEG6000,high salt and abscisic acid(ABA),and differential expression patterns of OsERF103 were showed under the different treatment conditions.This study provides basic data for further exploring the biological function of OsERF103.

关 键 词：水稻 乙烯应答因子(ERF) 生物信息学 亚细胞定位 表达分析 

分 类 号：S511[农业科学—作物学]