体外内皮细胞培养装置的研制与实验研究  

The Development and Experimental Study of Endothelial Cell Culture Device in vitro

作　　者：刘雨佳 丁皓[1,2] 张迎 施春迅 聂智超 刘浩宇 陈泽华 LIU Yujia;DING Hao;ZHANG Ying;SHI Chuxun;NIE Zhichao;LIU Haoyu;CHEN Zehua(School of Medical Instrument and Food Engineering,University of Shanghai for Science and Technology,Shanghai 200093,China;Shanghai University of Medicine&Health Sciences,Shanghai 201318,China)

机构地区：[1]上海理工大学医疗器械与食品学院,上海200093 [2]上海健康医学院,上海201318

出　　处：《医用生物力学》2019年第5期541-547,共7页Journal of Medical Biomechanics

基　　金：上海市自然科学基金项目(17ZR1413500)

摘　　要：目的研制一种创新性的体外内皮细胞培养装置,即基于血液动力学环境的血管内皮细胞体外培养装置,介绍内皮细胞体外培养装置的研制与实验研究。方法运用血液动力学理论与方法,在课题组现有研究基础上设计并研制内皮细胞体外动态培养系统,其流动环境中切应力、正应力和张应力同时存在。并从装置的研制背景、结构与组成、设计原理、理论基础以及实验研究5个方面详细描述该装置的研制和实验研究。结果装置能够准确模拟正常生理水平下内皮细胞所处的力学环境,实现切应力、正应力、张应力分别在0~12 Pa、0~15.96 k Pa、0~0.5 MPa范围内的精确调控。结论装置能提供一个更接近人体生理条件的血液动力学环境,为深入探索血管内膜损伤机制提供更理想的实验环境和手段。Objective To develop an innovative device for endothelial cell culture in vitro,namely,to develop a vascular endothelial cell culture device based on hemodynamic environment,so as to introduce the development and experimental study of endothelial cell culture device in vitro.Methods A device of dynamic culture system for endothelial cells in vitro on the basis of the existing research was designed with the theory and method of hemodynamics.The shear stress,positive stress and tensile stress existed at the same time in the flow environment.The development and experimental research of the device were described in detail from 5 aspects,such as the development background,structure and composition,design principle,theoretical basis and experimental research.Results The device could accurately simulate the hemodynamic environment of endothelial cells at normal level,with precise control of shear stress in 0-12 Pa range,positive stress in 0-15.96 kPa range,and tensile stress in 0-0.5 MPa range.Conclusions The device can provide a hemodynamic environment which is closer to the physiological conditions of human body,as well as a more ideal experimental environment and means for further exploring the mechanism of vascular intimal injury.

关 键 词：体外培养装置 内皮细胞 血液动力学 应力 

分 类 号：R318.01[医药卫生—生物医学工程]