快速膜乳化技术制备均一小粒径复合多糖微球及其色谱性能评价  

Preparation and Properties of Uniform Small-Sized Polysaccharide Composite Microspheres by Premix Membrane Emulsification Technique

作　　者：张洪武[1,2] 赵岚 黄永东[2] 朱凯[2] 朱天孝 李冬雪 张荣月 苏志国 马光辉[2] 王启宝[1] Zhang Hongwu;Zhao Lan;Huang Yongdong;Zhu Kai;Zhu Tianxiao;Li Dongxue;Zhang Rongyue;Su Zhiguo;Ma Guanghui;Wang Qibao(School of Chemical&Environmental Engineering,China University of Mining&Technology,Beijing 100083,China;State Key Laboratory of Biochemical Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing 100190,China;Department of Applied Chemistry,Beijing Institute of Petrochemical Technology,Beijing 102617,China)

机构地区：[1]中国矿业大学(北京)化学与环境工程学院,北京100083 [2]中国科学院过程工程研究所生化工程国家重点实验室,北京100190 [3]北京石油化工学院应用化学系,北京102617

出　　处：《化学世界》2019年第11期774-783,共10页Chemical World

基　　金：国家重点研发计划(Nos.2018YFC031101,2016YFF0202304);国家自然科学基金(No.21476241);中国科学院重点部署(No.KFZD-SW-218);北京市自然科学基金(No.2172054);北京市教委科技一般(No.KM201710017002)资助项目

摘　　要：采用快速膜乳化技术制备均一小粒径复合多糖微球,重点探究膜乳化工艺关键参数和微球固化冷却工艺等对微球形貌、粒径及其分布的影响,以及制备工艺对微球耐压性能的影响,在此基础上制备粒径均一、耐压性能良好的小粒径复合多糖微球。色谱研究表明,该均一小粒径复合多糖微球具有较高的色谱分离度,在高分辨率色谱领域具有良好的应用前景。Uniform small-sized polysaccharide composite microspheres were prepared by premix membrane emulsification technique followed by alternative cross-linking technique.Optical microscope,laser particle size analyzer and scanning electron microscope were used for characterization of microspheres.Effects of key parameters including both premix membrane emulsification process and cooling solidification process on the morphology,particle size and size distribution of microspheres were studied.The effect of preparation process on the pressure resistance property was also investigated.Under the optimized conditions,polysaccharide composite microspheres with uniform particle size and superior pressure resistance were prepared.Chromatography results showed that these uniform small-sized polysaccharide composite microspheres had good separation property so that they have potential application in high resolution chromatography.

关 键 词：快速膜乳化技术 复合多糖微球 粒径均一 高分辨率色谱 分离纯化 

分 类 号：O652[理学—分析化学] Q81[理学—化学]