优质鸡SESN1基因单核苷酸多态性及其与生长性状的关联分析  

Single Nucleotide Polymorphisms (SNPs) of Quality Chicken SESN1 and Association Analysis with Growth Trait

作　　者：马曼婷 陈小兰 蔡柏林 聂庆华[1,2] MA Manting;CHEN Xiaolan;CAI Bolin;NIE Qinghua(College of Animal Science,South China Agricultural University,Guangzhou,Guangdong 510642;Guangdong Provincial Key Lab of Agro-Animal Genomics and Molecular Breeding,Guangzhou,Guangdong 510642)

机构地区：[1]华南农业大学动物科学学院,广东广州510642 [2]广东省农业动物基因组学和分子育种重点实验室,广东广州510642

出　　处：《中国家禽》2019年第21期7-10,共4页China Poultry

基　　金：广东省科技计划项目(2018B020203001、2016LM1113)

摘　　要：为探究SESN1基因单核苷酸多态性与优质鸡生长性状的相关性,研究采用直接测序法扩增SESN1基因CDS区前2 000 bp调控区域,结果发现9个单核苷酸多态性位点。通过对这些位点进行连锁分析,划分为C-1365T^G-1543C、G-1177A^C-1294A、T-1058G^T-1076A组成的3个单倍型块。通过分析突变位点与温氏N301家系生长性状的相关性,发现位点C-1365T、T-1076A、G-1058T均与温氏N301家系胸肌重达显著相关(P<0.05)。结果表明,SESN1基因与家鸡肌肉生长密切相关,上述多态位点可作为研究肉鸡胸肌重指标的潜在分子标记。In order to study the correlation between the polymorphism of SESN1 gene and chicken growth traits,the 5′-flanking region of chicken SESN1 was amplified by direct sequencing, and a total of nine SNPs were identified.Three haplotype blocks consisting of C-1365 T^G-1543 C, G-1177 A^C-1294 A, and T-1058 G^T-1076 A, were divided by linkage analysis. Association analysis with growth traits found three SNPs(C-1365 T, T-1076 A and G-1058 T) were significantly associated with breast muscle weight(P<0.05) of Wenshi N301 chicken. The results suggested that SESN1 gene was highly associated with muscle growth, might play a crucial role in the growth and development of skeletal muscle.

关 键 词：SESN1基因 多态性位点 单倍型 胸肌重 鸡 

分 类 号：S831.2[农业科学—畜牧学]