一测多评法测定葛根药材中9个异黄酮成分的含量  

Determination of 9 isoflavonoids in Puerariae Lobatae Radix with quantitative analysis of multi-components by single marker

作　　者：木盼盼 安琪 张彦昭 郭龙 郭梅 郑玉光 张丹 MU Pan-pan;AN Qi;ZHANG Yan-zhao;GUO Long;GUO Mei;ZHENG Yu-guang;ZHANG Dan(College of Pharmacy,Hebei University of Chinese Medicine,Shijiazhuang 050200,China)

机构地区：[1]河北中医学院药学院

出　　处：《中国中药杂志》2019年第22期4888-4895,共8页China Journal of Chinese Materia Medica

基　　金：河北省自然科学基金青年项目(H2018423029);河北省高等学校科学技术研究项目(QN2016103);中央财政公共卫生专项“2017,2018年中药资源普查项目”(Z135080000022);全国中药炮制技术传承基地项目(冀中医药[2017]37号);河北省第二期现代农业产业技术体系创新团队项目(HBCT2018060205);河北省中医药管理局科研计划项目(2015079);河北省大学生创新创业计划训练项目(201814432049)

摘　　要：建立葛根中9个异黄酮含量测定的一测多评方法,并验证该方法的准确度及可行性。采用高效液相色谱法(HPLC),以葛根素为内标物,建立3’-羟基葛根素、葛根素芹菜糖苷、3’-甲氧基葛根素、葛根素-6″-O-木糖苷、大豆苷、染料木苷、芒柄花素和大豆苷元与葛根素的相对校正因子,计算与其他8种成分的质量分数。同时采用外标法和一测多评法分别测定11批样品中的9个异黄酮的含量,比较外标法与一测多评法的结果,以验证一测多评方法的准确性及可行性。实验结果表明,在不同的实验条件下相对校正因子重现性好,9个成分的外标法与一测多评法的含量测定结果无显著差异。因此,以葛根素为内标物,同时测定3’-羟基葛根素、葛根素芹菜糖苷、3’-甲氧基葛根素、葛根素-6″-O-木糖苷、大豆苷、染料木苷、芒柄花素和大豆苷元的一测多评法准确可行,可用于葛根药材的质量控制。A method for determination of 9 isoflavones in Puerariae Lobatae Radix was established and the accuracy and feasibility of the method were verified. The relative correction factors of eight isoflavonoids,3’-hydroxy puerarin,puerarinapioside,3’-methoxy puerarin,puerarin 6″-O-xyloside,daidzin,genistin,formononetin and daidzein were determined by HPLC method with puerarin as the internal standard. The contents of 9 isoflavonoids in 11 batches of samples were determined by external standard method and QAMS.The accuracy and feasibility of the methods were evaluated by comparison of the quantitative results between external standard method and QAMS. The reproducibility of the relative correction factors was good under different experimental conditions,and there was no significant difference between the external standard method of the 9 compounds and the content of QAMS method. The results showed that using puerarin as an internal standard to simultaneously determine the 8 isoflavonoids mentioned above is accurate and feasible. Thus,it can be used as quality control of Puerariae Lobatae Radix.

关 键 词：葛根 异黄酮 一测多评 外标法 相对校正因子 

分 类 号：R28[医药卫生—中药学]