炮制对葶苈子的指标成分在大鼠体内药代动力学的影响研究  

Effects of processing on pharmacokinetics of Descurainiae Semen’s constituents in rats

作　　者：于现阔 罗寒燕 鲁亚奇 张晓 吴宏伟[1] 唐力英[1] 王祝举[1] YU Xian-kuo;LUO Han-yan;LU Ya-qi;ZHANG Xiao;WU Hong-wei;TANG Li-ying;WANG Zhu-ju(Institute of Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China;College of Pharmacy,Henan University of Chinese Medicine,Zhengzhou 450046,China)

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]河南中医药大学药学院,河南郑州450046

出　　处：《中国中药杂志》2019年第22期4947-4952,共6页China Journal of Chinese Materia Medica

基　　金：国家自然科学基金面上项目(81673602)

摘　　要：首次建立了UPLC-MS/MS法同时测定大鼠血浆中槲皮素-3-O-β-D-葡萄糖-7-O-β-D-龙胆双糖苷(QGG)和芥子酸的浓度,并用于炮制对葶苈子中这2个药效成分的药代动力学影响研究。以沙苑子苷A为内标,血浆样品用甲醇沉淀蛋白,UPLC-MS/MS分析,方法经专属性、线性范围、准确度、精密度、提取回收率和基质效应、稳定性等确证该方法可靠,适用。分别灌胃给予等量生药量的生品或炮制品葶苈子60%乙醇提取物后,于不同时间点采血,测定血药浓度,采用DAS 3.2.6软件非房室模型法计算药代动力学参数,SPSS 23.0进行统计学分析,绘制血药浓度-时间曲线图。结果显示,炮制品组QGG各药代动力学参数较生品组无显著性差异,而芥子酸曲线下面积较生品组显著升高,平均驻留时间延长。结果表明,炮制有利于葶苈子中芥子酸的吸收,并减缓排泄时间,对QGG的吸收程度无明显影响。An UPLC-MS/MS method simultaneously determining contents of quercetin-3-O-β-D-glucose-7-O-β-D-gentiobioside and sinapic acid in rats’plasma was firstly established and applied to study the effects of processing on pharmacokinetics of Descurainiae Semen's active constituents.Complantatoside A as internal standard,methanol used for protein precipitation,the method was validated according to the instructions of CFDA.Rats'plasma was collected after being oral administrated equal dosage of 60%ethanal extract of raw or processed Descurainiae Semen at different point of time,then the concentrations were determined to calculate pharmacokinetic parameters using DAS 3.2.6.And the parameters were analyzed using SPSS 23.0,meantime the concentration-time curve was drawn.The results showed that processing had no effects on the pharmacokinetics of QGG,but could improve the absorption of sinapic acid and slow down the excretion.

关 键 词：南葶苈子 炮制 槲皮素-3-O-β-D-葡萄糖-7-O-β-D-龙胆双糖苷 芥子酸 超高效液相色谱-质谱联用法 药代动力学 

分 类 号：R28[医药卫生—中药学]