富血小板血浆-琼脂凝胶修复兔关节软骨缺损的实验研究  

Physical and chemical properties of bioactive scaffolds based on platelet rich plasma and the repair mechanism in promoting cartilage defects

作　　者：吴辉 郭明岗 李亮[1] 罗旭伟 侯铁奇 WU Hui;GUO Ming-gang;LI Liang;LUO Xu-wei;HOU Tie-qi(Department of Orthopedics,the first affiliated Hospital of University of South China,Hengyang,Hunan,421001,China)

机构地区：[1]南华大学附属第一医院骨外科

出　　处：《中国骨与关节杂志》2019年第11期862-867,共6页Chinese Journal of Bone and Joint

摘　　要：目的探讨基于富血小板血浆-琼脂糖凝胶支架制备方法、理化性能及在促进软骨缺损中的修复效果。方法(1)支架制备。购置7~9个月龄成年新西兰大白兔30只,10只用于软骨细胞的分离、培养,制备富血小板血浆,接种在琼脂糖凝胶及富血小板血浆中,制备琼脂糖凝胶支架及富血小板血浆-琼脂糖凝胶支架,测定支架的理化性能;(2)取剩余20只成年新西兰大白兔,建立股骨远端关节面骨软骨缺损模型,随机分为琼脂糖凝胶支架组(n=10)和复合支架组(n=10)。琼脂糖凝胶支架组采用琼脂糖凝胶支架进行修复,复合支架组采用富血小板血浆-琼脂糖凝胶支架修复,比较两组修复效果。结果两组实验细胞从第1~21天保持增长状态,细胞在两种支架上起初OD值差异无统计学意义(P>0.05);细胞培养7~21天细胞在复合支架组上增殖率,高于琼脂糖凝胶支架组(P<0.05);复合支架组在4周及8周支架中GAG、胶原及DNA含量,高于琼脂糖凝胶支架组(P<0.05);复合支架组修复后4周模型缺损部位得到愈合;修复后8周缺损部位修复,新生组织部位与正常组织无明显界限,光泽;琼脂糖凝胶支架组修复后4周缺损部位明显,但是缺损部位较小;修复后8周缺损部位愈合,但是与正常组织界限明显;复合支架组修复后4周支架部分降解,可见残余材料,与软骨下骨间间隙消失;修复后8周支架完全降解,高度与正常软骨组织一致,软骨下骨链接紧密,多以透明软骨细胞为主。琼脂糖凝胶支架组修复后4周支架开始降解,与周围软骨结合较好,但是软骨下骨存在较大间隙;修复后8周缺损部位得到修复,但是存在明显凹陷,无光泽。结论富血小板血浆-琼脂糖凝胶支架能促进缺损部位修复。Objective To explore preparation methods,physical and chemical properties of bioactive scaffolds based on platelet rich plasma,and to analyze the repair mechanism in promoting cartilage defects.Methods(1)Scaffold preparation:30 adult New Zealand white rabbits aged 7-9 months were purchased,and 10 were used for the isolation and culture of chondrocytes;platelet-rich plasma was prepared and inoculated in agarose gel and plateletrich plasma to prepare agarose gel scaffold and platelet-rich platelets;physical and chemical properties of scaffolds were determined;(2)Remaining 20 adult New Zealand white rabbits were used to establish a model of osteoarticular defect of the distal femoral articular surface.All were randomly divided into agarose gel scaffold group(n=10,repaired by agarose gel scaffold)and composite scaffold group(n=10,repaired by platelet-rich plasma-agarose gel scaffold).Repair effects of the two groups were compared.Results Experimental cells maintained growth from day 1 to day 21 with no significant differences in the initial OD values between the two groups(P>0.05).Cell increase rate of the composite scaffold group was higher than that of the agarose gel scaffold group when cultured for 7-21 days(P<0.05).The content of GAG,collagen and DNA in the composite scaffold group was higher than that of the agarose gel scaffold group at the 4 th and 8 th week(P<0.05).Composite scaffold group:defects healed after 4 weeks;defects were repaired after 8 weeks;no obvious boundary between the new tissues and the normal tissues;the gloss was obvious;scaffold partially degraded,subchondral bone space disappeared after 4 weeks;scaffold completely degraded,the height was consistent with normal cartilage tissues,the subchondral bone was closely linked mostly with hyaline chondrocytes after 8 weeks.Agarose gel scaffold group:less defects but still obvious after 4 weeks;defects healed after 8 weeks;obvious boundary between the new tissues and the normal tissues;scaffold began to degrade and bound well with the surrounding

关 键 词：富血小板血浆 琼脂糖凝胶支架 股骨远端关节面骨软骨缺损 修复效果 软骨细胞 

分 类 号：R681.3[医药卫生—骨科学]