梓树6个种源耐盐性差异的生物测定  

Bioassay for Salt Tolerance of Six Catalpa ovata Provenances

作　　者：李媛 李志辉[2] 苗晓娟[1] 王智 王军辉[1] 麻文俊[1] LI Yuan;LI Zhi-hui;MIAO Xiao-juan;WANG Zhi;WANG Jun-hui;MA Wen-jun(Research Institute of Forestry,Chinese Academy of Forestry,State Key Laboratory of Tree Genetics and Breeding,Key Laboratory of Tree Breeding and Cultivation,National Forestry and Grassland Administration,National Innovation Alliance of Catalapa bungei,Beijing 100091,China;Central South University of Forestry and Technology,Changsha 410004,Hunan,China)

机构地区：[1]中国林业科学研究院林业研究所,林木遗传育种国家重点实验室,国家林业和草原局林木培育重点实验室,楸树国家创新联盟,北京100091 [2]中南林业科技大学,湖南长沙410004

出　　处：《林业科学研究》2019年第6期106-114,共9页Forest Research

基　　金：中央级公益性科研院所基本科研业务费专项资金“楸树优质高抗良种选育研究”(CAFYBB2017ZA001-8)

摘　　要：[目的]为选择出抗盐性强的梓树种源作为楸树的嫁接砧木,拓展楸树的适生范围,旨在为选择耐盐梓树种源提供理论依据。[方法]以6个梓树种源的种子为材料,设置4个NaCl浓度梯度(0.0%、0.3%、0.6%、1.0%)开展种子萌发试验,观察其种子萌发状况,测定各胁迫处理20 d后芽苗生长表现、酶活性以及脯氨酸、MDA等可溶性物质的变化规律,计算各指标的相关指数选择评价指标,利用模糊数学中的隶属函数法结合权重综合评价各梓树种源的耐NaCl能力。[结果]6个梓树种源种子的长度、宽度、长宽比、种翅长、千粒质量以及在盐胁迫下的芽苗中的MDA、脯氨酸、可溶性糖、可溶性蛋白含量和SOD、POD、CAT活性均差异极显著,胚根、胚轴、幼苗的长度差异显著;各种源种子发芽的盐胁迫临界浓度(NaCl浓度)为0.6%;运用临界NaCl浓度下的指标数据进行综合评价得出梓树抗盐性排名,其耐NaCl性由大到小为辽宁桓仁、甘肃正宁、湖北襄阳、湖南吉首、贵州兴仁和河南洛阳。[结论]在盐渍土进行楸树嫁接苗繁育时,适宜选择辽宁恒仁种源梓树苗木作为其嫁接砧木。[Objective]The purpose of this study is to select salt-tolerance Catalpa ovata provenances as the rootstock for C.bungei grafting,so as to expand the range suitable of C.bungei growth and alleviate the tight domestic demand for high-quality C.ovata seedlings.[Method]The seeds of 6 C.ovata provenances were collected.NaCl treatments were performed on these seeds to simulate salt stress.The salt tolerance were analyzed by test seed germination and seedling growth.The provenances showed strong salt tolerance were selected as provenances suitable to be used as rootstock to graft seedlings of C.bungei.The variation of germination,enzyme activity and soluble substances such as proline and MDA in the seedlings after 20 days’NaCl treatments(4 concentrations:0%,0.3%,0.6%,1%)was measured.The correlation indexes of the data was calculated to select the proper evaluation indexes.Membership function method in fuzzy mathematics together with proportion was used to evaluate the NaCl tolerance of C.bungei from different provenances comprehensively.[Result]Under salt stress,the length,width,fin length and 1000 grain weight of the seeds,and the content of MDA,proline,soluble sugar and soluble protein and SOD,POD,CAT activity among these seedlings from the 6 provenances showed significant differences.The length of radicle,hypocotyl and seedlings was also significantly changed under salt stress.The threshold NaCl concentration for the germination of these seeds was 0.6%.The salt resistance of C.bungei was comprehensively evaluated using indexes under threshold NaCl concentration.[Conclusion]For breeding C.bungei grafted seedlings in saline soil,the provenance from Hengren of Liaoning Province is the most suitable for the rootstock seedlings of C.ovata,while the provenance from Luoyang of Henan Province is the least suitable provenance.

关 键 词：耐盐性 嫁接 种源 梓树 

分 类 号：S718.43[农业科学—林学]