中华稻蝗共生菌Fusarium sp.ZMT01固相培养代谢产物  

The metabolites by solid fermentation from the symbiotic fungus Fusarium sp. ZMT01 of Oxya chinensis Thunber

作　　者：陈嘉晴 朱水容 钟毓 李晓琪 张小琳 杨玉莹 何泽康 佘志刚[2] 李春远 CHEN Jiaqing;ZHU Shuirong;ZHONG Yu;LI Xiaoqi;ZHANG Xiaolin;YANG Yuying;HE Zekang;SHE Zhigang;LI Chunyuan(College of Materials and Energy,South China Agricultural University,Guangzhou 510642,China;School of Chemistry,Sun Yat-sen University,Guangzhou 510275,China)

机构地区：[1]华南农业大学材料与能源学院,广东广州510642 [2]中山大学化学学院,广东广州510275

出　　处：《中山大学学报（自然科学版）》2019年第6期14-18,共5页Acta Scientiarum Naturalium Universitatis Sunyatseni

基　　金：广东省自然科学基金(2018A030313582);广东省科技计划项目(2016A020222019);广州市科技计划项目(201707010342);华南农业大学大学生创新创业训练项目(201810564494,201810564499)

摘　　要：研究中华稻蝗肠道共生菌Fusariumsp.ZMT01固相发酵代谢产物,用现代层析技术从乙醇提取物中分离单体,波谱技术鉴定结构,二倍稀释法测试抗菌活性。分离到3-苯基-2-环戊烯-1-酮(1),2-羟基-3-苯基-2-环戊烯-1-酮(2),曲酸(3),曲酸单甲醚(4),(S)-5-羟基-2,6-二甲基-4H-呋喃[3,4-g]苯并吡喃-4,8(6H)-二酮(5),7,8-二甲基苯并蝶啶-2,4(1H,3H)-二酮(6),(9Z,11E)-8,13-二羟基-9,11-十八碳二烯酸(7),豆甾-7,22-二烯-3β,5α,6α-三醇(8)共8个化合物。化合物1,2是首次以天然产物形式分离到,化合物2的核磁数据为首次报道,化合物5~7为首次从Fusarium属分离到。化合物5对小麦赤霉菌、荔枝炭疽菌显示强抗菌活性。The metabolites by solid fermentation of the symbiotic fungus Fusarium sp.ZMT01 from the intestinal tract of Oxya chinensis Thunber were studied.The molecules were isolated from the ethanol extract by the modern chromatography technology.Their structures were elucidated by spectral methods.The antifungal activities were evaluated using the dilution method.Eight compounds were isolated,they are,3-phenylcyclopent-2-en-1-one(1),2-hydroxy-3-phenylcyclopent-2-en-1-one(2),kojic acid(3),kojic acid monomethyl ether(4),(S)-5-hydroxy-2,6-dimethyl-4 H-furo[3,4-g]benzopyran-4,8(6 H)-dione(5),7,8-dimethylbenzo[g]pteridine-2,4(1 H,3 H)-dione(6),(9 Z,11 E)-8,13-dihydroxyoctadeca-9,11-dienoic acid.(7)and stigma-7,22-dien-3β,5α,6α-triol(8).Among them,compounds 1 and 2 were first isolated as natural products.The 1H NMR and 13 C NMR data of compound 2 were also reported firstly.Compounds 5-7 were obtained from Fusarium sp.for the first time.Compound 5 exhibited high inhibitory activities against Fusarium graminearum Schw.and Colletotrichum gloeosporioides Penz..

关 键 词：昆虫共生菌 Fusariumsp 中华稻蝗 抗真菌活性 

分 类 号：O629.9[理学—有机化学]