不同时间艾灸预处理对卵巢储备功能减退大鼠卵巢功能的影响  

Effect of moxibustion pretreatment at different time on ovarian function in rats with diminished ovarian reserve

作　　者：金洵[1] 吕兴 韦艳会 朱世鹏 陈欢[3] JIN Xun;LU Xing;WEI Yan-hui;ZHU Shi-peng;CHEN Huan(The Second Clinical Medical College,Nanjing University of Chinese Medicine,Nanjing 210023,China;Central Research Institute of Shanghai Pharmaceuticals Holding Co.,Ltd,Shanghai 200020;Department of Acupuncture-moxibustion,the First Affiliated Hospital of Nanjing Medical University,Nanjing 210029)

机构地区：[1]南京中医药大学第二临床医学院,南京210023 [2]上海医药有限公司中央研究院,上海200020 [3]南京医科大学第一附属医院针灸科,南京210029

出　　处：《针刺研究》2019年第11期817-821,共5页Acupuncture Research

基　　金：国家自然科学基金青年基金项目(No.81603674、81804179);江苏省自然科学基金青年基金项目(No.BK20161049)

摘　　要：目的:观察不同时间艾灸预处理对卵巢储备功能减退(DOR)大鼠血清激素水平变化的影响,探讨艾灸预处理对卵巢储备功能保护效应的机制。方法:SD雌性大鼠随机分为空白组、模型组、艾灸1组、艾灸2组和艾灸3组,每组8只。用雷公藤混悬液灌胃复制DOR大鼠模型。艾灸各组予"肾俞""关元"麦粒灸治疗,每穴7壮,每日1次,每周5次,共治疗4周。艾灸1组于干预4周后造模,艾灸2组艾灸干预2周后造模同时再予艾灸干预2周,艾灸3组造模同时艾灸2周,造模结束后继续艾灸2周。检测各组大鼠体质量、卵巢指数,酶联免疫吸附法检测血清抗苗勒管激素(AMH)、卵泡刺激素(FSH)、雌二醇(E2)、雄激素(T)、脱氢表雄酮(DHEA)含量变化。结果:各组大鼠体质量无明显差异(P>0. 05)。模型组大鼠卵巢指数低于空白组(P<0. 05),艾灸2组和艾灸3组大鼠卵巢指数较模型组升高,但差异无统计学意义(P>0. 05)。模型组大鼠血清AMH水平低于空白组(P<0. 05),艾灸2组和艾灸3组血清AMH水平较模型组升高(P<0. 05)。模型组大鼠血清FSH、E2、T、DHEA水平高于空白组(P<0. 05);各艾灸组血清FSH水平均较模型组显著降低(P<0. 05),艾灸2组低于艾灸1组和艾灸3组(P<0. 05);艾灸2组和艾灸3组血清E2、T水平较模型组降低(P<0. 05),艾灸2组低于艾灸3组(P<0. 05);各艾灸组血清DHEA水平均较模型组降低(P<0. 05)。结论:艾灸预处理可改善DOR模型大鼠的卵巢储备功能,但艾灸介入时间不同,效应不同,疾病前配合疾病早期干预效应最佳,其次为疾病早期。单纯疾病前干预对卵巢储备能力的保护效应虽然不明显,但对DHEA却有显著影响。Objective To observe the effect of moxibustion pretreatment at different time on serum hormone levels in diminished ovarian reserve(DOR)rats,so as to explore its protective mechanisms.MethodsForty female SD rats were randomly divided into control,model,moxibustion-1(moxibustion was given 4 weeks before modeling),moxibustion-2(moxibustion was given 2 weeks before modeling and 2 weeks from the 1 st day on after modeling)and moxibustion-3(moxibustion was given 4 weeks from the 1 st day on after modeling)groups(n=8 rats in each group). The DOR model was established by gavage of Tripterygium Glycosides(75 mg/kg)once daily for 14 days. Grain-moxibustion was applied to"Shenshu"(BL23)and"Guanyuan"(CV4)for 7 cones,5 times a week for 4 weeks. The body weight and the ovary weight were recorded for calculating the ovarian index. The levels of serum anti-mullerian hormone(AMH),follicle stimulating hormone(FSH),estradiol(E2),androgen(T)and dehydroepiandrosterone(DHEA)were detected by ELISA.ResultsAfter modeling,ovarian index and serum AMH levels were obviously decreased(P<0. 05),and the levels of serum FSH,E2,T and DHEA were significantly increased in contrast with the control group(P < 0. 05).Following intervention and compared with the model group,the serum FSH and DHEA levels of each moxibustion group were significantly reduced(P<0. 05),the AMH levels significantly increased and E2 and T contents significantly decreased in the moxibustion-2 and moxibustion-3 groups(P<0. 05). The serum FSH,E2 and T contents in moxibustion-2 group were obviously lower than those of the moxibustion-1 and moxibustion-3 groups(P<0. 05).ConclusionMoxibustion pre-treatment can improve ovarian reserve function in DOR rats,while the effect is different with different intervention time,and the best intervention time is pre-occurrence and early stage of DOR.

关 键 词：艾灸预处理 卵巢储备功能减退 时效关系 

分 类 号：R245.8[医药卫生—针灸推拿学]