朱砂安神丸肝肾解毒物质基础与作用机制研究  

Study on substance basis and mechanism of detoxification in liver and kidney of Zhusha Anshen Pills

作　　者：王启铭 肖珅 翟菲[1] 梁建坤[1] 肖同美 项荣武[1] WANG Qi-ming;XIAO Shen;ZHAI Fei;LIANG Jian-kun;XIAO Tong-mei;XIANG Rong-wu(Shenyang Pharmaceutical University,Shenyang 110016,China)

机构地区：[1]沈阳药科大学

出　　处：《中华中医药杂志》2019年第11期5103-5111,共9页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：辽宁省科学技术计划项目(No.20170540850);辽宁省教育厅基本科研项目(No.2017LFW02)~~

摘　　要：目的:采用网络药理学的方法探讨朱砂安神丸肝肾解毒物质基础与作用机制。方法:通过TCMSP等数据库,结合口服生物利用度(OB)和类药性(DL)值,筛选出黄连、地黄、当归、甘草的化学成分,并使用PubChem数据库进行靶点预测,通过文献检索获取朱砂肝肾毒性的靶点;构建蛋白互作网络(PPI网络),寻找与朱砂靶点有直接相互作用的靶点;最后进行GO及KEGG通路分析和成分-靶点-通路网络分析。结果:最终得到成分122个,朱砂靶点35个,中药靶点288个,其中与朱砂有相互作用的靶点49个,通路分析结果中经文献验证具有解毒作用的通路共5条。结论:网络分析结果表明,朱砂安神丸中对肝肾毒性有解毒作用的主要为黄连和甘草,解毒成分有槲皮素、阿魏酸、甘草酸、甘草次酸、甘草苷等。Objective:A network pharmacology method was adopted to explore the substance basis and mechanism of the detoxification in liver and kidney of Zhusha Anshen Pills.Methods:Firstly,TCMSP and other databases were searched,and then the data were combined with oral bioavailability and drug-likeness analysis to screen the chemical constituents of Rhizoma Coptidis,Radix Rehmanniae Preparata,Radix Angelicae Sinensis,Radix Glycyrrhizae.Target of compounds was predicted by using Pubchem database.The targets of liver and kidney toxicity in cinnabar was completed through literature search.Then build a PPI network to find targets that interact directly with cinnabar targets.Finally,GO and KEGG pathway analysis and compoundtarget-pathway network analysis were performed.Results:In the end,there were 122 compounds,35 targets of cinnabar,288 targets of Chinese materia medica,49 targets of which had interactions with cinnabar,and 5 pathways for detoxification were verified through literature analysis.Conclusion:The results of network analysis showed that the detoxification in liver and kidney of Zhusha Anshen Pills were mainly Rhizoma Coptidis and Radix Glycyrrhizae,and the detoxification compounds were quercetin,ferulic acid,glycyrrhizic acid,glycyrrhetinic acid,and glycyrrhizin,et al.

关 键 词：朱砂安神丸 网络药理学 解毒 物质基础 

分 类 号：R28[医药卫生—中药学]