环介导恒温扩增法快速检测曲霉菌的研究  

Detection of Aspergillus by loop-mediated isothermal amplification

作　　者：章白苓[1] 徐轶[2] 杭亚平[1] 张楠[1] 邹珊 ZHANG Bai-ling;XU Yi;Hang YaPing;Zhang Nan;Zou Shan(The Second Affiliated Hospital of Nanchang University,Nanchang 30000 China;Jiangxi Provincil People'sHospital,Nanchang 330006,China)

机构地区：[1]南昌大学第二附属医院检验科,江西南昌330006 [2]江西省人民医院,江西南昌330006

出　　处：《实验与检验医学》2019年第6期995-997,共3页Experimental and Laboratory Medicine

基　　金：江西省卫生计生委科技计划，编号:20175284;江西省科技厅重点研发计划，编号：20161BBG70124

摘　　要：目的建立环介导恒温扩增(LAMP)检测曲霉菌的方法。方法用LAMP技术扩增曲霉菌菌株,并应用临床标本检测,同时对方法特异性、敏感性进行观察。结果所测曲霉菌菌株均获扩增产物,对其他非曲霉菌菌株无交叉反应,其检测敏感性可达0.05pg。结论LAMP方法特异性好,灵敏度高,操作简便,快速,适合从临床标本中检测曲霉菌。Objective To establish loop-mediated isothemal amplification for detecting Aspergillus.Methods Aspergillus strains were amplified by LAMP and detected by clinical samples.The specificity and sensitivity of the method were observed.Results all Aspergillus strains were amplified and there was no cross reaction to other non-Aspergillus strains.The sensitivity of LAMP was up to 0.05pg.Conclusion LAMP is of strong specificity,high sensitivity,and is convenient,fast,as well as suitable for the detection of aspergillus from clinical specimens.

关 键 词：环介导恒温扩增技术(LAMP) 曲霉菌 检测 

分 类 号：R379.6[医药卫生—病原生物学] Q78[医药卫生—基础医学]