环状RNA在慢性阻塞性肺疾病诊断及预后判断中的研究价值  

Value of serum circular ribonucleic acid in diagnosis and prognosis in chronic obstructive pulmonary diseases

作　　者：陈福涛[1] 钟富宽[1] 白巧红 卞光荣[1] 杨飏[1] 朱江[1] Chen Futao;Zhong Fukuan;Bai Qiaohong;Bian Guangrong;Yang Yang;Zhu Jiang(Department of respiration,The Second Hospital of Lianyungang,Lianyugang 222000,China;Department of respiratory,The Second Hospital of Nanjing,Nanjing 210003,China)

机构地区：[1]江苏省连云港市第二人民医院呼吸科,江苏连云港222000 [2]南京中医药大学附属南京医院(南京市第二医院)呼吸科,江苏南京210003

出　　处：《中华卫生应急电子杂志》2019年第5期285-291,共7页Chinese Journal of Hygiene Rescue(Electronic Edition)

基　　金：连云港市卫健委科研项目(201718)

摘　　要：目的探讨血清环状核糖核酸(circRNA)在慢性阻塞性肺病(COPD)诊断及预后判断中的研究价值。方法选择连云港市第二人民医院2014年1月至2016年12月收治的80例COPD患者以及同期在该院进行健康体检人群30例为研究对象进行回顾性随机对照研究,其中男性58例,女性52例;年龄61~82岁,平均(70±6)岁。通过Arraystar circRNA芯片筛选获得两组受试者血清样本中差异表达的circRNA,再利用qRT-PCR验证了获得的目标circRNA分子,并结合生物信息学软件对其进行注释和功能预测。结果本研究对circRNA-001988、circRNA-0000344和circRNA-0000284在COPD组和正常组血清中的表达的差异进行了探究:发现与正常组相比,circRNA-001988和circRNA-0000344在COPD组血清样本中的表达分别下调了(6.54±2.75)倍和(5.94±2.89)倍;circRNA-0000284则在COPD组的血清样本中上调表达了(8.54±3.25)倍。差异均有统计学意义(P<0.05)。结论血清circRNA可作为COPD诊断和预后判断的生物标志物,具有较好的临床预测价值。Objective To study the value of the serum circular ribonucleic acid(circRNA)in the diagnosis and prognosis in of chronic obstructive pulmonary disease(COPD). Methods From January 2014 to December 2016, 80 patients with COPD were selected from Lianyungang Second People’s Hospital, and 30 patients who underwent health check-ups in the hospital during the same period were selected as randomized control The varying expression of circRNA in the serum samples of two groups of subjects was obtained by using the Arraystar circRNA chip screening, the acquired target circRNA molecule was were validated using qRT-PCR, and the annotation and functional prediction were used in combination with bioinformatics software. Results The differences in expression in circRNA-001988, circRNA-0000344 and circRNA-0000284 in COPD group and normal group serum were explored in this study: compared to normal groups, the expression of circRNA-001988 and circRNA-0000344 was reduced in serum samples in the COPD group by(6.54±2.75) and(5.94±2.89)t imes, respectively, and circRNA-0000284 was increased in serum samples in the COPD group by(8.54±3.25) times. The difference was statistically significant(P<0.05). Conclusion Serum circRNA can be used as a biomarker for COPD diagnosis and prognosis, and has good clinical predictive value.

关 键 词：环状核糖核酸 慢性阻塞性肺病 诊断 预测价值 

分 类 号：R56[医药卫生—呼吸系统]