ACC氧化酶反义基因转化‘翠玉’猕猴桃  

Transformation of ’Cuiyu’ Kiwifruit with ACC Oxidase Antisense Gene

作　　者：苑平 田宏现[2] 杨莉颖[3] 王曼玲 吴娟娟[1] 孔佑涵 Yuan Ping;Tian Hongxian;Yang Liying;Wang Manling;Wu Juanjuan;Kong Youhan(Hunan Horticulture Research Institute,Hunan Academy of Agricultural Sciences,Changsha,410125;Biological Resources and Environmental Sciences,Jishou University,Jishou,416000;Hunan Vegetables Research Institute,Hunan Academy of Agricultural Sciences,Changsha,410125;Key Laboratory of Agroecological Processes in Subtropical Region,Institute of Subtropical Agriculture,Chinese Academy of Sciences,Changsha,410125)

机构地区：[1]湖南省农业科学院,湖南省园艺研究所,长沙410125 [2]吉首大学生物资源与环境科学学院,416000 [3]湖南省农业科学院,湖南省蔬菜研究所,长沙410125 [4]中国科学院亚热带农业生态研究所,亚热带农业生态过程重点实验室,长沙410125

出　　处：《分子植物育种》2019年第22期7390-7394,共5页Molecular Plant Breeding

基　　金：湖南省农业科技创新资金项目(2018ZD09);湖南省农业科技创新资金(2018QN22);湖南省自然科学基金(2017JJ3159);湖南省重点研发计划项目(2018NK2011)共同资助

摘　　要：为建立‘翠玉’猕猴桃基因功能研究技术平台并通过生物技术改良‘翠玉’猕猴桃,本研究以‘翠玉’猕猴桃叶片为实验材料,利用农杆菌介导法将ACC氧化酶(ACO)基因的反义链导入‘翠玉’猕猴桃,共获得14株潮霉素抗性植株。PCR鉴定结果显示,其中8株扩增获得了目的条带,初步统计阳性植株约为57.1%。随机挑选其中4株进行实时荧光定量PCR检测,结果表明,ACO基因的相对表达量是野生型植株的22%~41%。本研究为利用分子生物学手段改良‘翠玉’猕猴桃品种提供了工作基础。In order to establish the technology platform for function analysis of genes and genetic improvement of ‘Cuiyu’ kiwifruit through biotechnology,the leaves of ‘Cuiyu’ Kiwifruit plants were used as experimental materials in this study.By using Actinidia delicious-mediated method,the antisense expression vector of ACC Oxidase(ACO)gene was successfully transformed ‘Cuiyu’ Kiwifruit,and positive transgenic plants were identified.A total of 14 hygromycin resistant plants were obtained.PCR identification results showed that 8 strains obtained the target bands by amplification,and the preliminary positive plants were about 57.1%.Four plants were randomly selected for real-time quantitative PCR detection.The results showed that the relative expression of ACO gene was 22%~41% of that of wild plants.This research provides a working basis for using molecular biology methods to improve of‘Cui yu’ kiwifruit.

关 键 词：翠玉猕猴桃 反义RNA技术 农杆菌 组织培养 遗传转化 

分 类 号：S66[农业科学—果树学]