蓬子菜水溶液治疗糖尿病性大鼠慢性皮肤溃疡的效果及其最佳治疗浓度研究  

Study on the curative effect and the best concentration of Galium verum L. Solution on chronic skin ulcer in diabetic rats

作　　者：苑海刚 赵钢[2] 贾振[2] 吕勃川[2] 黄艳红[2] 张百亮[2] 姜德友[2] 王学军[1] YUAN Haigang;ZHAO Gang;JIA Zhen;LYU Bochuan;HUANG Yanhong;ZHANG Bailiang;JIANG Deyou;WANG Xuejun(Heilongjiang Academy of Traditional Chinese Medicine,Heilongjiang Province,Harbin 150036,China;Department of Peripheral Vascular Disease,the First Affiliated Hospital of Heilongjiang University of Chinese Medicine,Heilongjiang Province,Harbin 150040,China)

机构地区：[1]黑龙江省中医药科学院,黑龙江哈尔滨150036 [2]黑龙江中医药大学附属第一医院周围血管病科,黑龙江哈尔滨150040

出　　处：《中国医药导报》2019年第31期26-29,共4页China Medical Herald

基　　金：黑龙江省中医药管理局重点项目(ZHY18-002)

摘　　要：目的探讨中药蓬子菜水溶液对糖尿病性大鼠慢性皮肤溃疡愈合的疗效及最佳治疗浓度。方法选取60只健康的Wistar雄性大鼠,链脲佐菌素腹腔注射法建立糖尿病模型,应用随机数字表法将Wistar大鼠分为6组:高浓度(30 mg/mL)、中浓度(3 mg/mL)、低浓度蓬子菜治疗组(0.3 mg/mL)和阴性对照组、模型对照组、贝复济组,每组10只。造模后2 d开始治疗,创面常规换药基础上蓬子菜治疗组分别喷洒相应浓度的蓬子菜水溶液,阴性对照组喷洒生理盐水,贝复济组喷洒贝复济(浓度为4200 U/mL)进行治疗。造模后3、7、10 d透明生物膜描膜法计算创面剩余面积。造模后第10天处死大鼠,取局部创面组织行HE染色,观察病理组织学改变。结果各组溃疡面均随时间延长逐渐缩小,同一时间点的各浓度蓬子菜治疗组和贝复济组创面面积明显小于阴性对照组和模型对照组,差异有统计学意义(P<0.05);同一时间点各浓度蓬子菜治疗组创面面积明显小于贝复济组,中浓度蓬子菜治疗组创面缩小最为明显,差异有统计学意义(P<0.05)。不同处理因素与时间因素间存在交互作用(P<0.05)。贝复济组创面修复优于阴性对照组和模型对照组,应用不同浓度蓬子菜水溶液治疗的各组均优于贝复济组,其中中浓度蓬子菜治疗组创面愈合修复最好,差异有统计学意义(P<0.05)。结论蓬子菜水溶液治疗大鼠皮肤溃疡在不同时间点与贝复济比较更有效。蓬子菜水溶液有效促进大鼠溃疡创面愈合的最佳治疗浓度为3 mg/mL。Objective To explore the curative effect and the best therapeutic concentration of Galium verum L. Solution on chronic skin ulcer in diabetic rats. Methods Sixty healthy male Wistar rats were selected to establish diabetic model by intraperitoneal injection of streptozotocin. Random number table method was used to divided the Wistar rats into six groups: high concentration group(30 mg/m L), medium concentration group(3 mg/m L), low concentration group(0.3 mg/m L), negative control group, model control group and Befuji group, 10 per group. Treatment was taken on the second day after modeling. On the basis of routine dressing change on the wound, the treatment groups of the scutellaria chinensis were sprayed with the corresponding concentration of Galium verum L. Solution. The negative control group was sprayed with normal saline, while the Befuji group was sprayed with Befuji(concentration was 4200 U/m L). The transparent membrane was used to tracing film along the ulcer edge and the software was taken to calculate the remaining area on the third, seventh and tenth day after modeling. All the rats were sacrificed and local wound tissues were stained with HE to observe the pathological changes on the tenth day after modeling. Results The ulcer surface of each group decreased gradually with time. The wound areas of various concentrations of Galium verum L.groups and the Beifuji group were significantly smallerthan those of the negative control group and the model control group at the same time point, and the differences were statistically significant(P < 0.05). The wound area of various concentrations of Galium verum L. groups were significantly smaller than those of the Beifuji group at the same time point, while the wound area of medium concentration Galium verum L. group decreased most obviously, and the differences were statistically significant(P < 0.05). There are interactions between different processing factors and time factors(P < 0.05). The wound repair of beifuji group was better than that of negative con

关 键 词：蓬子菜水溶液 慢性皮肤溃疡 贝复济 透明生物膜 

分 类 号：R285.5[医药卫生—中药学]