丹酚酸B对代谢记忆环境下RSC96细胞的保护作用  

The protective effects of salvianolic acid B on RSC96 cell in metabolic memory environment

作　　者：王蒙[1] 王倩倩 孙连庆[2] WANG Meng;WANG Qianqian;SUN Lianqing(Xi’an Jiaotong University,Xi’an 710049)

机构地区：[1]西安交通大学,西安710049 [2]西安交通大学第一附属医院,西安710061

出　　处：《陕西医学杂志》2019年第12期1587-1590,共4页Shaanxi Medical Journal

基　　金：国家自然科学基金资助项目(81673785);陕西省自然科学基金资助重点项目(2015JZ023)

摘　　要：目的:探讨丹酚酸B对代谢记忆环境下离体培养的RSC96细胞凋亡及炎性因子变化规律的影响。方法:RSC96细胞分别培养于低糖培养组(5.6 mmol/L)、代谢记忆(125 mmol/L高糖培养48 h,5.6 mmol/L低糖培养24 h)、丹酚酸B组(125 mmol/L高糖+1μmol/L丹酚酸B培养48 h,5.6 mmol/L低糖培养基+1μmol/L丹酚酸B培养24h)中,流式法检测ROS含量,Western blot法检测Cleaved-caspase 9,Cleaved-caspase 3,P65,IL-1β的表达。结果:代谢记忆环境可下调ROS含量,下调Cleaved-caspase 9,Cleaved-caspase 3,P65,IL-1β等蛋白的表达;丹酚酸B可上调ROS含量,下调Cleaved-caspase 9,Cleaved-caspase 3,P65,IL-1β等蛋白的表达。结论:代谢记忆环境下,丹酚酸B可能通过下调ROS引起细胞凋亡及炎性损伤保护RSC96细胞。Objective:To observe the effect of Sal B on apoptosis and inflammatory of RSC96 cultured in metabolic memory environment.Methods:RSC96 cells were respectively cultured in control group(5.6 mmol/L),metabolic memory(125 mmol/L high glucose for 48 h plus 5.6 mmol/L glucose for 24 h) or Sal B group(125 mmol/L high glucose + 1μmol/L Sal B for 48 h plus 5.6 mmol/L glucose+ 1μmol/L Sal B for 24 h).Flow cytometry was performed to detect ROS level.Western blot was manipulated to detect the expression of Cleaved-caspase 9,Cleaved-caspase 3,P65,IL-1β.Results:The metabolic memory group ROS level was up-regulated compared with the control group.However,Sal B group ROS level was down-regulated compared with the metabolic memory group.The expression of Cleaved-caspase 9,Cleaved-caspase 3,P65,IL-1β in metabolic memory group decreased compared with the control group.However,the expression of Cleaved-caspase 9,Cleaved-caspase 3,P65,IL-1β in Sal B decreased compared with the metabolic memory group.Conclusion:Sal B protected RSC96 cells against apoptosis and inflammatory by eliminating ROS in metabolic memory environment.

关 键 词：凋亡 炎症 RSC96 代谢记忆 丹酚酸B 活性氧 

分 类 号：R392.5[医药卫生—免疫学]