甘蓝型油菜每角粒数的全基因组关联分析  

Genome-wide association study of seed number per silique in rapeseed(Brassica napus L.)

作　　者：孙程明 陈锋[1] 陈松[1] 彭琦[1] 张维[1] 易斌[2] 张洁夫[1] 傅廷栋[2] SUN Cheng-Ming;CHEN Feng;CHEN Song;PENG Qi;ZHANG Wei;YI Bin;ZHANG Jie-Fu;FU Ting-Dong(Institute of Industrial Crops,Jiangsu Academy of Agricultural Sciences/Key Laboratory of Cotton and Rapeseed(Nanjing),Ministry of Agriculture/Jiangsu Collaborative Innovation Center for Modern Crop Production,Nanjing 210014,Jiangsu,China;National Key Laboratory of Crop Genetic Improvement/College of Plant Science and Technology,Huazhong Agricultural University,Wuhan 430070,Hubei,China)

机构地区：[1]江苏省农业科学院经济作物研究所/农业部长江下游棉花与油菜重点实验室/江苏省现代作物生产协同创新中心,江苏南京210014 [2]华中农业大学植物科学技术学院/作物遗传改良国家重点实验室,湖北武汉430070

出　　处：《作物学报》2020年第1期147-153,I0002-I0010,共16页Acta Agronomica Sinica

基　　金：国家重点研发计划项目(2018YFD0100602);国家现代农业产业技术体系建设专项(CARS-12);江苏省农业科技自主创新基金(CX(19)3055);江苏省基础研究计划(自然科学基金)项目(BK20190260);中央高校基本科研业务费专项资金资助项(2662016PY063)资助~~

摘　　要：每角粒数是油菜重要的产量构成因子,增加每角粒数有助于提高油菜的籽粒产量。利用Illumina 60K SNP芯片对496份具有代表性的油菜资源进行基因型分析,考察该群体在2个环境中的每角粒数,利用MLM和GLM模型进行全基因组关联分析。结果表明,本群体在2个环境中每角粒数的广义遗传力为57.7%。利用MLM和GLM模型分别检测到9个和20个位点,所有MLM位点均得到GLM结果的验证。6个位点与前人定位的QTL重叠,其中2个位点得到2次验证,其余14个是新位点。在7个位点附近找到了候选基因,其中在C09染色体的位点Bn-scaff155761-p74980附近找到已克隆的油菜每角粒数基因BnaC9.SMG7b,在其余6个位点附近找到GRDP1、SPATULA、HVA22D、DA2等已知的拟南芥每角粒数基因的同源基因。本研究结果有助于解析油菜每角粒数的遗传基础及其调控机制,为每角粒数的遗传改良奠定了基础。Seed number per silique(SSN) is a key component of seed yield in rapeseed, increasing SSN can improve the seed yield of plants. A collection of 496 representative rapeseed accessions was genotyped by the Illumina 60 K SNP array and phenotyped for SSN in two environments. The genome-wide association study(GWAS) of SSN was performed via the MLM(Mixed linear model) and GLM(General linear model). The broad-sense heritability of SSN was 57.7%. Nine and twenty loci were detected with MLM and GLM, respectively, and all loci detected by MLM were included those by GLM. Six loci were overlapped with reported QTLs, and two of them were validated by two independent researches, and the rest 14 loci were new. We identified plausible candidate genes nearby seven loci, and the reported rapeseed SSN gene BnaC9.SMG7 b was found near the locus Bn-scaff155761-p74980 on C09 chromosome detected in this study. Besides, six candidates orthologous to documented Arabidopsis SSN genes, like GRDP1, SPATULA, HVA22 D, and DA2, were found near our GWAS loci. The results provide an insight into the genetic basis of seed number per silique and lay a foundation for further mechanism exploration and breeding for this trait in B. napus.

关 键 词：甘蓝型油菜 产量 每角粒数 关联分析 SNP标记 

分 类 号：S51[农业科学—作物学]