Pyramiding disease resistance genes in elite winter wheat germplasm for Western Canada  

作　　者：AndréLaroche Michele Frick Robert J.Graf Jamie Larsen John D.Laurie 

机构地区：[1]Agriculture and Agri-Food Canada,Lethbridge Research and Development Centre,Lethbridge,Alberta T1J 4B1,Canada [2]Agriculture and Agri-Food Canada,Harrow Research and Development Centre,2585 County Road 20,Harrow,Ontario N0R 1G0,Canada

出　　处：《The Crop Journal》2019年第6期739-749,共11页作物学报（英文版）

基　　金：Funding from the Agriculture and Agri-Food Canada Peer Review and Growing Forward programs,and Ducks Unlimited Canada is greatly appreciated

摘　　要：We report on pyramiding different disease resistance genes against fungal pathogens in Canadian winter wheat germplasm based on available DNA markers and gene sequences.Genetic resistance represents a safe, economical and ecological method for protecting plants, growers and the health of consumers. Major diseases of wheat on the Canadian Prairies are common bunt, rusts(leaf, stem and stripe) and Fusarium head blight. Over the years markers for resistance genes against these diseases have been identified and used by the international wheat community. We describe markers that we have used to pyramid different resistance genes and indicate their presence in Canadian winter wheat cultivars issued from the winter wheat breeding program at the Agriculture and Agri-Food Canada,Lethbridge Research and Development Centre, the only winter wheat breeding program in Western Canada actively delivering new varieties for all regions of the Canadian Prairies.The sources of resistance and identities of PCR primers and amplification conditions are indicated to enable the transfer and pyramiding of different resistance(R) genes to breeding lines. We conclude by reviewing new tools for identifying R genes in wheat and indicate how mutagenesis and gene editing can help future efforts to extend the protection offered by known R genes.We report on pyramiding different disease resistance genes against fungal pathogens in Canadian winter wheat germplasm based on available DNA markers and gene sequences.Genetic resistance represents a safe, economical and ecological method for protecting plants, growers and the health of consumers. Major diseases of wheat on the Canadian Prairies are common bunt, rusts(leaf, stem and stripe) and Fusarium head blight. Over the years markers for resistance genes against these diseases have been identified and used by the international wheat community. We describe markers that we have used to pyramid different resistance genes and indicate their presence in Canadian winter wheat cultivars issued from the winter wheat breeding program at the Agriculture and Agri-Food Canada,Lethbridge Research and Development Centre, the only winter wheat breeding program in Western Canada actively delivering new varieties for all regions of the Canadian Prairies.The sources of resistance and identities of PCR primers and amplification conditions are indicated to enable the transfer and pyramiding of different resistance(R) genes to breeding lines. We conclude by reviewing new tools for identifying R genes in wheat and indicate how mutagenesis and gene editing can help future efforts to extend the protection offered by known R genes.

关 键 词：Bunt Gene PYRAMIDING Wheat rusts Marker-assisted selection TRITICUM AESTIVUM 

分 类 号：S51[农业科学—作物学]