Meta-analysis of QTL for Fusarium head blight resistance in Chinese wheat landraces  

作　　者：Jin Cai Shan Wang Zhenqi Su Tao Li Xianghui Zhang Guihua Bai 

机构地区：[1]Institute of Germplasm Resources and Biotechnology and Jiangsu Provincial Key Laboratory of Agrobiology,Jiangsu Academy of Agricultural Sciences,Nanjing 210014,Jiangsu,China [2]Department of Agronomy,Kansas State University,Manhattan,KS 66506,USA [3]Institute of Cereal and Oil Crops,Hebei Academy of Agricultural and Forestry Sciences,Shijiazhuang 050035,Hebei,China [4]Jiangsu Provincial Key Laboratory of Crop Genetics and Physiology,Yangzhou University,Yangzhou 225009,Jiangsu,China [5]College of Plant Science,Jilin University,Changchun 130062,Jilin,China [6]United State Department of Agriculture-Agricultural Research Service,Hard Winter Wheat Genetics Research Unit,Manhattan,KS 66506,USA

出　　处：《The Crop Journal》2019年第6期784-798,共15页作物学报（英文版）

基　　金：partly funded by US Wheat and Barley Scab Initiative and the National Research Initiative Competitive Grants (2017-6700725939, 2017-67007-25929) from the National Institute of Food and Agriculture, U.S. Department of Agriculture;from Natural Science Foundation of Jiangsu Province, China (BK20170596);Jiangsu Agricultural Science and Technology Innovation Fund, China (CX (17) 3004)

摘　　要：Epidemics of Fusarium head blight(FHB), incited by Fusarium graminearum Schwabe, in wheat cause significant reductions in grain yield and quality. Numerous quantitative trait loci(QTL) for FHB resistance have been reported from Chinese sources. However, the relationships among QTL from different landraces have not been characterized. We earlier mapped QTL for FHB resistance using low-density maps developed from five recombinant inbred line(RIL) populations involving Chinese landraces ‘Haiyanzhong’(HYZ),‘Wangshuibai’(WSB), ‘Baishanyuehuang’(BSYH), ‘Huangfangzhu’(HFZ), and‘Huangcandou’(HCD) as FHB resistant parents. In this study, we used maps of single nucleotide polymorphisms(SNP) developed from the five populations and identified 31 QTL on 16 chromosomes;10 QTL were new. We constructed a consensus map and identified six meta-QTL(MQTL) and SNP within the MQTL regions using meta-analysis. Two of the MQTL were on chromosome 3 BS(3 BSd and 3 BSc), and one on each of chromosomes 3A, 2D, 3D,and 4D. Twenty-two SNP closely linked to MQTL were converted into breeder friendly Kompetitive Allele Specific Polymerase Chain Reaction(KASP) assays, which should be useful for marker-assisted selection in breeding programs.Epidemics of Fusarium head blight(FHB), incited by Fusarium graminearum Schwabe, in wheat cause significant reductions in grain yield and quality. Numerous quantitative trait loci(QTL) for FHB resistance have been reported from Chinese sources. However, the relationships among QTL from different landraces have not been characterized. We earlier mapped QTL for FHB resistance using low-density maps developed from five recombinant inbred line(RIL) populations involving Chinese landraces ‘Haiyanzhong’(HYZ),‘Wangshuibai’(WSB), ‘Baishanyuehuang’(BSYH), ‘Huangfangzhu’(HFZ), and‘Huangcandou’(HCD) as FHB resistant parents. In this study, we used maps of single nucleotide polymorphisms(SNP) developed from the five populations and identified 31 QTL on 16 chromosomes; 10 QTL were new. We constructed a consensus map and identified six meta-QTL(MQTL) and SNP within the MQTL regions using meta-analysis. Two of the MQTL were on chromosome 3 BS(3 BSd and 3 BSc), and one on each of chromosomes 3 A, 2 D, 3 D,and 4 D. Twenty-two SNP closely linked to MQTL were converted into breeder friendly Kompetitive Allele Specific Polymerase Chain Reaction(KASP) assays, which should be useful for marker-assisted selection in breeding programs.

关 键 词：Genotyping-by-sequencing FHB RESISTANCE KASP META-ANALYSIS of QTL Single nucleotide polymorphism TRITICUM AESTIVUM 

分 类 号：S51[农业科学—作物学]