引发对老化大麦种子可溶性物质及抗氧化酶活性的影响  

Effects of Seed Priming on Soluble Substances Content and Antioxidant Enzymes Activities in Aged Barley Grains

作　　者：朱迎树 齐军仓[1] 李剑 赵准 李忠豪[1] 宋瑞娇 韩欣梦 李虎情 方梁 ZHU Yingshu;QI Juncang;LI Jian;ZHAO Zhun;LI Zhonghao;SONG Ruijiao;HAN Xinmeng;LI Huqing;FANG Liang(College of Agronomy,Shihezi University,Shihezi Xinjiang 832003,China)

机构地区：[1]石河子大学农学院

出　　处：《种子》2019年第11期29-33,共5页Seed

基　　金：现代农业产业技术体系建设专项(CARS-05-22 B)

摘　　要：为探讨聚乙二醇(PEG)和氯化钙(CaCl2)溶液引发对老化大麦种子活力的影响,本研究采用人工加速老化法对大麦种子进行处理,以人工老化程度为高活力、中活力、低活力的大麦种子为试材,采用PEG和CaCl2引发的最佳方式(与对照差异极显著)进行处理,并分析种子胚中可溶性糖含量和可溶性蛋白含量以及SOD、POD和CAT活性变化的趋势。结果表明:引发显著增加了老化大麦种子中可溶性糖含量和可溶性蛋白含量,显著提高了SOD、POD和CAT酶活性,而且随吸胀时间变化种子中贮藏物质含量和部分酶活性的变化趋势各不相同,在不同的引发方式中,20%PEG和10 mg·L^-1 CaCl2引发24 h效果最显著。这表明种子引发能够修复种子老化损伤和提高种子萌发过程中的酶活性。To explore the effects of polyethylene glycol(PEG)and calcium chloride(CaCl2)solution-mediated priming action on the seed vigor of aging barley,the barley seeds were firstly treated with artificial accelerated aging method and these seeds with characteristics of high aging,moderate aging and low-degree aging were selected as plant materials for further study respectively.After that,these seeds of different degrees of aging were triggered by PEG and CaCl2 and soluble sugar content,soluble protein content and the activities of SOD,POD,CAT in embryo were determined.The results showed that the trigger action caused a significant increase in the content soluble sugar and soluble protein,also in activities of SOD,POD and CAT.Meanwhile,the content of storage substances and some enzyme activities in seeds varied with the time of imbibition.Among these differently trigged ways,the priming action with 20%PEG and 10 mg·L^-1 CaCl2 caused the most significant effect,which indicated the trigger action could restore protective enzyme activity in artificial aging seeds during germination.

关 键 词：大麦 种子引发 贮藏物质 抗氧化酶 

分 类 号：S512.3[农业科学—作物学] S311