低温胁迫对大果榉枝条抗寒性的影响  

Cold Resistance of Zelkova sinica under Low Temperature Stress

作　　者：陈博[1] 石进朝[1] 李迎春 CHEN Bo;SHI Jin-chao;LI Ying-chun(Department of Horticulture,Beijing Vocational College of Agriculture,Beijing 102442,P.R.China;Huangfa Nursery,Beijing 102604,P.R.China)

机构地区：[1]北京农业职业学院园艺系,北京102442 [2]黄垡苗圃,北京102604

出　　处：《西部林业科学》2019年第6期35-40,共6页Journal of West China Forestry Science

基　　金：2018北京园林绿化增彩延绿科技创新工程科学研究项目(XM-0000154102180424058)

摘　　要：为掌握北京地区引种栽培大果榉的抗寒特性,以垂枝榆为对照,6年生大果榉为试材,选取其一年生休眠枝条分别在5℃(CK)、-10℃、-15℃、-20℃、-25℃、-30℃和-35℃下进行低温处理,测定枝条的相对电导率,应用Logistic方程拟合半致死温度(LT 50);并测定其枝条的丙二醛(MDA)和可溶性糖的含量,过氧化物酶(POD)和过氧化氢酶(CAT)的活性,枝条恢复生长后的萌芽率,研究低温胁迫下大果榉休眠枝条的抗寒性。结果表明:随温度降低,相对电导率整体呈上升趋势;MDA、可溶性糖的含量以及POD和CAT酶活性均呈现先上升后下降的趋势;恢复生长率鉴定植物抗寒能力的方法易受到植物扦插成活难易程度的影响;相关性最高的抗寒指标是相对电导率,其次是MDA和可溶性糖。大果榉和垂枝榆的LT 50分别为-33.11℃和-29.83℃,大果榉的抗寒性高于垂枝榆。This paper represents results from a focused test by co-authors for fully understanding the Zelkova sinica’s cold resistance properties and inform decision-making regarding introduction of the species for cultivation in Beijing.In the test,a number of one-year-old dormant branches of the Z.sinica species and Ulmus pumila cv.species(as a frame of reference)were collected for testing under artificial low temperatures(i.e.,5℃,-10℃,-15℃,-20℃,-25℃,-30℃and-35℃respectively),where relative electrical conductivity of the branches and the species’fitting semi-lethal temperatures(LT 50)from logistic equation were determined.Also determined in the process were concentrations of malondialdehyde(MDA)and soluble sugar,activity of peroxidase(POD)and catalase(CAT),as well as the species’in-season germination rate.The test in question indicates that the relative electrical conductivity of the Z.sinica species increases in general for all time with the decrease of temperature,while the concentrations of MDA and soluble sugar,and the activity of POD and CAT show an increase-and-decrease trend under the same circumstances;Techniques for identifying the plant’s levels of resistance to cold are prone to be affected by levels of difficulty involved in the cutting and planting process;Most relevant indicators for the plant’s resistance to cold temperatures are relative electrical conductivity and concentrations of MDA and soluble sugar;The Z.sinica plant’s resistance to cold was stronger than that of the U.pumila cv.Pendula’s,as exemplified by their LT 50 figures(i.e.,-33.11℃vs.-29.83℃)obtained in the process.

关 键 词：大果榉 垂枝榆 低温胁迫 抗寒性 

分 类 号：S792.99[农业科学—林木遗传育种]