水曲柳FmNAC072基因克隆及表达模式分析  

Cloning and Expression Analysis of FmNAC072 Gene from Fraxinus mandshurica

作　　者：刘悦 于磊[1,2] 赵兴堂[1,2] 梁楠松[1,2] 詹亚光[1,2] LIU Yue;YU Lei;ZHAO Xing-tang;LIANG Nan-song;ZHAN Ya-guang(School of Life Science,Northeast Forestry University,Harbin Heilongjiang 150040,P.R.China;State Key Laboratory of Tree Genetics and Breeding,Northeast Forestry University,Harbin Heilongjiang 150040,P.R.China)

机构地区：[1]东北林业大学生命科学学院,黑龙江哈尔滨150040 [2]东北林业大学林木遗传育种国家重点实验室,黑龙江哈尔滨150040

出　　处：《西部林业科学》2019年第6期155-162,共8页Journal of West China Forestry Science

基　　金：黑龙江省应用技术研究与开发计划项目(GA19B201)

摘　　要：研究水曲柳FmNAC072基因在非生物胁迫及激素信号诱导下的表达特征,为深入分析FmNAC072基因功能提供参考。以水曲柳叶片为材料,用PCR法克隆目的基因,采用生物信息学软件分析其分子结构特征。通过荧光定量PCR研究FmNAC072基因在低温、盐非生物胁迫及ABA、IAA、GA 3、JA及SA激素诱导下的表达模式,并分析其时空表达特异性。结果表明,水曲柳NAC072基因序列为FmNAC072。该基因全长1011bp,含完整的开放阅读框,编码336个氨基酸。生物信息学分析表明FmNAC072为亲水性稳定蛋白,α-螺旋、延伸链和无规则卷曲分布整个蛋白,亚细胞定位预测在细胞核中,与樟子松的蛋白序列具有很高同源性。非生物胁迫处理及激素诱导下,FmNAC072基因表达量与对照组相比差异显著。时空表达检测中,FmNAC072基因在根表达量最高,5月份FmNAC072基因表达量达峰值。FmNAC072基因参与植物生长发育及逆境胁迫的平衡。The expression characteristics of FmNAC072 gene in Fraxinus mandshurica under abiotic stress and hormone signal induction were studied in order to provide reference for further analysis of the function of FmNAC072 gene.The target gene was cloned from leaves of Fraxinus mandshurica by PCR,its molecular structure was analyzed by bioinformatics software,and the expression pattern of FmNAC072 gene under low temperature,salt abiotic stress and induced by ABA,IAA,GA 3,JA and SA hormone was studied by real-time fluorescence quantitative PCR.The specificity of its spatiotemporal expression was analyzed.We had cloned NAC072 gene named FmNAC072.The gene is 1011bp,containing a complete open reading frame,encodes 336 amino acids.Bioinformatics analysis shows FmNAC072 is a hydrophilic stable protein.Alpha helix,Extended strand,Random coil distribution of the entire protein,subcellular localization prediction in the nucleus.It has high homology with the protein sequence of Olea europaea var.sylvestris.Under abiotic stress and hormone induction,the expression of FmNAC072 gene was significantly different from that of control group.The expression of FmNAC072 gene was the highest in root and reached its peak in May.FmNAC072 gene is involved in the balance of growing development and stress response.

关 键 词：水曲柳 FmNAC072 生物信息学分析 基因克隆 

分 类 号：S718.46[农业科学—林学] S792.41