急性肾损伤患者血清miR-21、miR-155水平变化观察  

Changes in levels of serum miR-21 and miR-155 in patients with acute kidney injury

作　　者：林建[1] 叶晴 詹林达[1] 菅宏蕴[1] LIN Jian;YE Qing;ZHAN Linda;JIAN Hongyun(Zhongshan People's Hospital,Zhongshan 528403,China)

机构地区：[1]中山市人民医院

出　　处：《山东医药》2019年第33期27-30,共4页Shandong Medical Journal

基　　金：广东省自然科学基金资助项目(2017A030310199)

摘　　要：目的观察急性肾损伤(AKI)患者血清miR-21、miR-155水平变化,并探讨其对预后的预测价值。方法AKI患者189例,根据患者治疗后1年内的临床结局分为存活组(157例)和死亡组(32例),并选取同期体检健康者116例作为对照组。采用PCR法检测入院时血清miR-21和miR-155,采用逐步多元线性回归分析AKI发生的影响因素,采用ROC分析血清miR-21和miR-155对AKI的预后预测价值。结果存活组、死亡组、对照组血清miR-21水平分别为2.45±0.48、3.81±0.92、1.69±0.47,miR-155水平分别为1.72±0.33、2.49±0.81、1.13±0.41,三组两两比较,P均<0.05。入院时血清miR-21(OR=2.041,95%CI=1.133~8.541,P=0.003)、miR-155(OR=1.349,95%CI=1.075~5.241,P=0.012)水平是AKI预后的影响因素。入院时血清miR-21和miR155对AKI患者预后的ROC AUC分别为0.828(95%CI:0.744~0.862)、0.796(95%CI:0.743~0.853),灵敏度分别为86.3%、81.7%。血清miR-21联合miR-155的ROC AUC为0.903(95%CI:0.842~0.957),灵敏度为91.6%。结论AKI患者血清miR-21、miR-155水平升高,入院时血清miR-21、miR-155水平是AKI预后的影响因素,二者水平的检测可预测患者的预后,以联合预测效果最佳。Objective To observe the changes of serum miR-21 and miR-155 levels in patients with acute kidney injury(AKI),and to explore their prognostic value.Methods Totally 189 patients with AKI were divided into the survival group(157 cases)and death group(32 cases)according to the clinical outcome within one year after treatment,and 116 healthy people in the same period were selected as the control group.PCR was used to detect the serum miR-21 and miR-155 at admission,stepwise multiple linear regression was used to analyze the influencing factors of AKI,and ROC curve was used to analyze the prognostic value of serum miR-21 and miR-155 for AKI.Results The levels of serum miR-21 in the survival group,death group and control group were 2.45±0.48,3.81±0.92,and 1.69±0.47,miR-155 were 1.72±0.33,2.49±0.81,and 1.13±0.41,respectively.There were significant differences between the three groups(all P<0.05).The serum levels of miR-21(OR=2.041,95%CI=1.133-8.541,P=0.003)and miR-155(OR=1.349,95%CI=1.075-5.241,P=0.012)were the influencing factors for prognosis of AKI.The AUC of ROC curve of serum miR-21 and miR-155 for the prognosis of AKI patients was 0.828(95%CI:0.744-0.862),0.796(95%CI:0.743-0.853),and the sensitivity was 86.3%and 81.7%,respectively.The AUC of the ROC curve of serum miR-21 combined with miR-155 for the prognosis of AKI patients was 0.903(95%CI:0.842-0.957)and the sensitivity was 91.6%.Conclusion The serum levels of miR-21 and miR-155 in patients with AKI increase,the serum levels of miR-21 and miR-155 at admission are the influencing factors of AKI prognosis,and the combined detection of them can predict the prognosis of patients,and the combined prediction is the best.

关 键 词：微小核糖核酸 微小核糖核酸-21 微小核糖核酸-155 肾损伤 急性肾损伤 

分 类 号：R459.5[医药卫生—治疗学]